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安全信息

D8515

Sigma-Aldrich

脱氧核糖核酸-纤维素双链 来源于小牛胸腺 DNA

lyophilized powder

别名:

ds-DNA-纤维素, 脱氧核糖核酸, DNA-纤维素

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About This Item

MDL编号:
MFCD00130925
UNSPSC代码:
41106305
eCl@ss:
32160414
NACRES:
NA.51

形式

lyophilized powder

质量水平

200

标记范围

3-8 mg double-stranded calf thymus DNA/g

储存温度

−20°C

相关类别

一般描述

来自小牛胸腺DNA的脱氧核醣核酸−纤维素双链，也称为ctDNA，是一种DNA天然来源，专为DNA结合剂研究而设计。ctDNA检出限在2.0ng左右，光散射强度（I(LS)）的线性范围为0.05-1.5 mµg。

应用

来自小牛胸腺DNA的脱氧核醣核酸−纤维素双链用于研究多种蛋白质、核苷酸和化学试剂（包括抗癌剂）的结合参数和结合特异性。 它经常用于下拉试验和色谱分析程序。

质量

可能含有微量的 Tris 和 EDTA。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What buffer do you use to swell the Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA (DNA Cellulose) resin, Product D8515?

    DNA-Cellulose can be swollen in 10 mM tris, 1 mM tetrasodium EDTA buffer (pH 7.9).  One gram of DNA-Cellulose will swell to approximately 4 mL of wet resin.

  4. How do you store Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA (DNA Cellulose) resin, Product D8515, once it is swollen?

    DNA-Cellulose can be stored swollen in 10 mM tris, 1 mM tetrasodium EDTA buffer (pH 7.5 - 8) for several months at 4°C (without a bacteriostat).

  5. Can Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA (DNA Cellulose), Product D8515, be exposed to high salt?

    DNA-Cellulose is stable in up to 5M NaCl.  DNA-Cellulose should not be exposed to solutions of NaOH, since NaOH denatures DNA from double-stranded to single-stranded.

  6. How do you regenerate the column using Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA (DNA Cellulose), Product D8515?

    A suggested procedure for the regeneration of a DNA-Cellulose column would be to use 5 - 10 column volumes of 2M NaCl in buffer, followed by the same volume of buffer alone.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. Can Product D8515, Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA, be used for purification of DNA-dependent RNA Polymerase?

    We have not tested Product D8515, Deoxyribonucleic acid-cellulose double-stranded from calf thymus DNA for this application. However, the following references utilizes double-stranded DNA cellulose for purification of DNA-dependent RNA Polymerase:J. of Bacteriology, 172, 5624-5630 (1990)Biochem. J.,133, 201-203 (1973)

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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T Kuzuhara et al.
Biochemical and biophysical research communications, 362(4), 805-810 (2007-09-04)
Tumor necrosis factor-alpha (TNF-alpha) inducing protein (Tipalpha) is a carcinogenic factor secreted from Helicobacter pylori (H. pylori), mediated through both enhanced expression of TNF-alpha and chemokine genes and activation of nuclear factor-kappaB. Since Tipalpha enters gastric cancer cells, the Tipalpha
D Pisetsky et al.
The Journal of rheumatology, 26(9), 1934-1938 (1999-09-24)
To elucidate the epitope structure to DNA by identifying antigenic determinants on bacterial DNA bound by anti-DNA antibodies from normal human subjects (NHS) and patients with systemic lupus erythematosus (SLE). Sera from NHS and patients with SLE were tested by
Liyun Zhang et al.
Scientific reports, 4, 3776-3776 (2014-01-21)
The development of a fluorescent probe capable of detecting and distinguishing the wide diversity of G-quadruplex structures is particularly challenging. Herein, we report a novel BODIPY-based fluorescent sensor (GQR) that shows unprecedented selectivity to parallel-stranded G-quadruplexes with exposed ends and
A Georgaki et al.
Nucleic acids research, 22(7), 1128-1134 (1994-04-11)
A DNA helicase from calf thymus, called DNA helicase F, copurified with replication protein A through several steps of purification including DEAE-Sephacel, hydroxyapatite and single stranded DNA cellulose. It is finally separated from replication protein A on FPLC Mono Q
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