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文件

D8388

Sigma-Aldrich

庚二酰亚胺酸二甲酯二盐酸盐 二盐酸盐

powder

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别名:
DMP, Dimethyl pimelinediimidate 二盐酸盐
线性分子式:
[CH3OC(=NH)CH2CH2]2CH2 · 2HCl
CAS号:
58537-94-3
分子量:
259.17
EC 号:
261-320-6
MDL编号:
MFCD00038942
UNSPSC代码:
12352200
PubChem化学物质编号:
24894208
NACRES:
NA.25

形式

powder

质量水平

200

反应适用性

reagent type: cross-linking reagent

溶解性

H2O: 10 mg/mL

储存温度

−20°C

SMILES字符串

Cl.Cl.COC(=N)CCCCCC(=N)OC

InChI

1S/C9H18N2O2.2ClH/c1-12-8(10)6-4-3-5-7-9(11)13-2;;/h10-11H,3-7H2,1-2H3;2*1H

InChI key

LRHXBHUTQWIZTN-UHFFFAOYSA-N

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一般描述

庚二亚氨酸二甲酯二盐酸盐是一种同双功能交联剂，适用于交联、蛋白相互作用和蛋白修饰。通常，在pH7.0-10.0的范围内，与伯胺反应形成脒键。

应用

庚二亚氨酸二甲酯二盐酸盐可用于开发一种直接从人类粪便样品中分离高纯度比氏肠胞（Enterocytozoon bieneusi）孢子的技术，为研究这种寄生虫开创出新的方法。

注意

该试剂在中性pH下易于水解。

其他说明

注意，脒键保留了原始的伯胺正电荷。 包含七个原子连接子。
2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

象形图

Exclamation mark
GHS07

警示用语：

Warning

危险声明

H315

危险分类

Skin Irrit. 2

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

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G B Karlsson et al.
Analytical biochemistry, 199(2), 219-222 (1991-12-01)
A method is described for the use of magnetic beads as a solid phase for the immunoprecipitation of labeled proteins. The anti-human transferrin receptor monoclonal antibody OKT-9 has been coupled to sheep anti-mouse IgG1-coated magnetic beads using the crosslinking agent
I Accoceberry et al.
Journal of clinical microbiology, 39(5), 1947-1951 (2001-04-28)
An original, reliable, and reproducible method for the purification of Enterocytozoon bieneusi spores from human stools is described. We recently reported the production of a species-specific monoclonal antibody (MAb) 6E52D9 immunoglobulin G2a (IgG2a) raised against the exospore of E. bieneusi
Kevin Michael Boehm et al.
BMC bioinformatics, 20(1), 7-7 (2019-01-07)
To further our understanding of immunopeptidomics, improved tools are needed to identify peptides presented by major histocompatibility complex class I (MHC-I). Many existing tools are limited by their reliance upon chemical affinity data, which is less biologically relevant than sampling
K Brew et al.
The Journal of biological chemistry, 250(4), 1434-1444 (1975-02-25)
The cross-linking of the two components of lactose synthetase, alpha-lactalbumin and a galactosyltransferase, with dimethylpimelimidate was examined. The extent of the cross-linking at pH 8.1 was found to be dependent upon the presence of substrates or inhibitors for the galactosyltransferase.
Semisynthetic fluorohydrolases prepared by chemical modification of ribonuclease.
David, E., et al.
Enz. Microbiol. Technol., 14, 885-892 (1992)
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