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安全信息

D8037

Sigma-Aldrich

Driselase 崩溃酶, 来自担子菌属

BioReagent, suitable for plant cell culture

别名:

Driselase崩溃酶 来源于担子菌

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About This Item

CAS号:
EC 号:
MDL编号:
UNSPSC代码:
10171502
NACRES:
NA.72

产品线

BioReagent

质量水平

表单

powder

组成

Protein, ≥10% biuret

技术

cell culture | plant: suitable

应用

agriculture

储存温度

−20°C

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应用

崩溃酶 担子菌属已用于:

  • Coccomyxa细胞的球质体制备
  • 基于CRISPR/Cas9的诱变方案,用于短柄草及其异源多倍体亲族Brachypodium hybridum
  • 用于细胞壁消化,以对莲藕根组织进行全标本包埋免疫定位

生化/生理作用

崩溃酶具有天然混合酶活性(真菌碳水化合物酶),可用于消化植物细胞壁以促进植物材料的浸渍、原生质体形成和提取过程。崩溃酶可释放细胞壁内的碳水化合物。本配方包含纤维素、内切-1,3-β-葡聚糖酶和木聚糖酶的酶活性。

其他说明

粗体粉末中含有昆布多糖酶、木聚糖酶和纤维素酶。

法律信息

Driselase is a trademark of ASKA Animal Health Co. Ltd.

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Dieter Hackenberg et al.
Plant physiology, 172(2), 1154-1166 (2016-08-24)
In this study, we report the functional characterization of heterotrimeric G-proteins from a nonvascular plant, the moss Physcomitrella patens. In plants, G-proteins have been characterized from only a few angiosperms to date, where their involvement has been shown during regulation
M C Ralet et al.
Carbohydrate research, 263(2), 227-241 (1994-10-17)
Cell walls from sugar-beet pulp contain some feruloyl groups linked to the pectic neutral side-chains. Enzymic as well as chemical hydrolysis of the pulp yielded a series of feruloylated oligosaccharides, which have been purified by Sephadex LH-20 and Biogel P-2
Laura A Moody et al.
The New phytologist, 218(3), 1270-1277 (2018-03-03)
Forward genetics is now straightforward in the moss Physcomitrella patens, and large mutant populations can be screened relatively easily. However, perturbation of development before the formation of gametes currently leaves no route to gene discovery. Somatic hybridization has previously been
T Ishii et al.
Carbohydrate research, 248, 179-190 (1993-10-04)
Hydrolysis of spinach-leaf cell walls with Driselase (a fungal enzyme preparation) released two arabino-oligosaccharides and one galactobiose, each carrying a ferulic acid moiety. The oligosaccharides were characterized by NMR spectroscopy, methylation analysis, and FABMS. They were O-(2-O-trans-feruloyl-alpha-L-arabinofuranosyl)-(1-->5)-L-arabinof uranose, O-(6-O-trans-feruloyl-beta-D-galactopyranosyl)-(1-->4)-D-galactopy ranose
D T Kaplan et al.
Journal of nematology, 22(3), 399-406 (1990-07-01)
Radopholus spp. were reared in carrot tissue culture via established procedures, with slight modification. Several plant tissue maceration enzymes and flotation media (salts and sucrose) were evaluated with regard to nematode toxicity and extraction efficiency. Best extraction of viable nematodes

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