产品线
BioReagent
质量水平
形式
powder
组成
Protein, ≥10% biuret
技术
cell culture | plant: suitable
应用
agriculture
储存温度
−20°C
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应用
崩溃酶™ 担子菌属已用于：
- Coccomyxa细胞的球质体制备
- 基于CRISPR/Cas9的诱变方案，用于短柄草及其异源多倍体亲族Brachypodium hybridum
- 用于细胞壁消化，以对莲藕根组织进行全标本包埋免疫定位
生化/生理作用
崩溃酶™具有天然混合酶活性（真菌碳水化合物酶），可用于消化植物细胞壁以促进植物材料的浸渍、原生质体形成和提取过程。崩溃酶可释放细胞壁内的碳水化合物。本配方包含纤维素、内切-1,3-β-葡聚糖酶和木聚糖酶的酶活性。
其他说明
粗体粉末中含有昆布多糖酶、木聚糖酶和纤维素酶。
法律信息
Driselase is a trademark of ASKA Animal Health Co. Ltd.
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
D P Blowers et al.
Plant physiology, 86(2), 505-509 (1988-02-01)
Plasma membrane vesicles from wild carrot cells grown in suspension culture were isolated by aqueous two-phase partitioning, and ATP-dependent phosphorylation was measured with [gamma-(32)P]ATP in the presence and absence of calcium. Treatment of the carrot cells with the cell wall
D T Kaplan et al.
Journal of nematology, 22(3), 399-406 (1990-07-01)
Radopholus spp. were reared in carrot tissue culture via established procedures, with slight modification. Several plant tissue maceration enzymes and flotation media (salts and sucrose) were evaluated with regard to nematode toxicity and extraction efficiency. Best extraction of viable nematodes
T Ishii et al.
Carbohydrate research, 248, 179-190 (1993-10-04)
Hydrolysis of spinach-leaf cell walls with Driselase (a fungal enzyme preparation) released two arabino-oligosaccharides and one galactobiose, each carrying a ferulic acid moiety. The oligosaccharides were characterized by NMR spectroscopy, methylation analysis, and FABMS. They were O-(2-O-trans-feruloyl-alpha-L-arabinofuranosyl)-(1-->5)-L-arabinof uranose, O-(6-O-trans-feruloyl-beta-D-galactopyranosyl)-(1-->4)-D-galactopy ranose
M C Ralet et al.
Carbohydrate research, 263(2), 227-241 (1994-10-17)
Cell walls from sugar-beet pulp contain some feruloyl groups linked to the pectic neutral side-chains. Enzymic as well as chemical hydrolysis of the pulp yielded a series of feruloylated oligosaccharides, which have been purified by Sephadex LH-20 and Biogel P-2
Dieter Hackenberg et al.
Plant physiology, 172(2), 1154-1166 (2016-08-24)
In this study, we report the functional characterization of heterotrimeric G-proteins from a nonvascular plant, the moss Physcomitrella patens. In plants, G-proteins have been characterized from only a few angiosperms to date, where their involvement has been shown during regulation
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