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生物来源
Porcine kidney
质量水平
形式
solid
比活
≥0.05 unit/mg solid
分子量
170 kDa
溶解性
100 mM sodium phosphate buffer, pH 7.2: soluble 10 mg/mL
异质活性
monoamine oxidase (benzylamine substrate) ≤1%
储存温度
−20°C
一般描述
猪肾来源的二胺氧化酶是由两个分子量均为87kDa的相同亚基组成的同二聚体，每个亚基含有一个吡哆醛磷酸分子和一个铜原子。酶分子量为170kDa。酶是一种糖蛋白，含有5%己糖、3.3%葡萄糖胺、2.6% N-乙酰葡萄糖胺和0.25% N-乙酰神经氨酸。酶对刀豆蛋白A具有高亲和力。以尸胺和组胺作为底物时的最适pH为6.3-7.4。
应用
来自猪肾的二胺氧化酶已被用于研究测定鸟氨酸脱羧酶活性的基于发光的测试。来自猪肾的二胺氧化酶也用于研究二胺氧化酶中 N-连接寡糖结构。
猪肾来源的二胺氧化酶已用于制备组胺生物传感器。
生化/生理作用
二胺氧化酶催化单胺、二胺和组胺氧化成为醛、氨和过氧化氢。该酶属于含铜的胺氧化酶，是氮代谢中的关键酶。二胺氧化酶受到二乙基二硫代氨基甲酸、苯肼、氨基脲、氰化物、异烟酸肼抑制。
单位定义
在 pH 7.2 和 37℃ 条件下，1 个单位每小时将氧化 1.0 μmol 腐胺。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
Y Huang et al.
Carbohydrate research, 323(1-4), 111-125 (2000-04-27)
Structures of the N-linked glycans released from porcine kidney diamine oxidase (DAO) were characterized utilizing various analytical techniques, including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI/TOF-MS), high-performance capillary electrophoresis (HPCE), and high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The
A Rinaldi et al.
Preparative biochemistry, 12(1), 11-28 (1982-01-01)
Several methods for the isolation of apparently homogeneous pig kidney diamine oxidase have been reported in recent years (1-7), but these procedures allow to obtain only little amounts of material making very difficult the study of the molecular properties of
Diamine oxidase and catalase are expressed in the same cells but are present in different subcellular compartments in porcine kidney.
H G Schwelberger et al.
Inflammation research : official journal of the European Histamine Research Society ... [et al.], 48 Suppl 1, S81-S82 (1999-06-01)
A PRELIMINARY INVESTIGATION ON A HISTAMINE BIOSENSOR CONSTRUCTED FROM DIAMINE OXIDASE IMMOBILISED ONTO AN OXYGEN PROBE
The Enzymes (1970)
Marta Moniente et al.
Food research international (Ottawa, Ont.), 160, 111735-111735 (2022-09-10)
Lentilactobacillus parabuchneri is the main bacteria responsible for the accumulation of histamine in cheese. The goal of this study was to assess the efficiency of potential histamine-degrading microbial strains or, alternatively, the action of the diamine oxidase (DAO) enzyme in
实验方案
To standardize a procedure for the enzymatic assay of Diamine Oxidase.
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