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此商品 | GERPN2106 | GERPN2209 | GERPN2109 |
|---|---|---|---|
| color Detection reagent 1: Colorless Detection reagent 2: Colorless | color Detection reagent 1: Colorless Detection reagent 2: Colorless | color Detection reagent 1: Colorless Detection reagent 2: Colorless | color Detection reagent 1: Colorless Detection reagent 2: Colorless |
| feature wetted part: no | feature wetted part: no | feature wetted part: no | feature wetted part: no |
| packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of 1 kit |
| manufacturer/tradename Cytiva RPN2134 | manufacturer/tradename Cytiva RPN2106 | manufacturer/tradename Cytiva RPN2209 | manufacturer/tradename Cytiva RPN2109 |
一般描述
MTP™ Taq DNA聚合酶是一种来自生栖热菌的重组热稳定酶，在大肠杆菌中表达并使用专有工艺纯化，以最大程度减少污染性 DNA 的水平。该酶同时具有 5′→3′ DNA 聚合酶核酸外切酶活性，据SDS-PAGE分析为∼95 kDa，它没有可检出的核酸内切酶或3′→5′ 核酸外切酶活性。每批 MTP Taq均经过严格的质量控制测试，以确保没有可检出水平的污染性DNA。
大多数其它聚合酶制剂中存在的污染性 DNA 常常会妨碍或掩盖结果的准确解释，尤其是在靶向保守序列时，例如细菌 16S rRNA 区域。通过 Sigma′专有的 DNA 去除方法和严格的质量控制标准，我们可以确保产品中不存在最常见的污染性 DNA。每批MTP Taq均使用PCR和对细菌16S rRNA的保守区域、 Taq表达载体和人β-肌动蛋白基因有特异性的引物进行分析。
虽然 MTP Taq 确保以高质量、低污染的 DNA 聚合酶进行可靠的 PCR 扩增，但许多其它试剂可以将 DNA 污染物引入 PCR。为了进一步降低 PCR 过程中污染物 DNA 的风险，我们在每管 MTP TaqDNA聚合酶中加入了10× MTP Taq 缓冲液。每批10× MTP Taq缓冲液均经过与MTP Taq DNA 相同的严格质量控制测试，以确保没有可检出水平的污染性DNA。为防止假阳性 PCR 结果，在以MTP Taq DNA 聚合酶进行的 PCR 反应中只能使用不含 DNA 的试剂。
大多数其它聚合酶制剂中存在的污染性 DNA 常常会妨碍或掩盖结果的准确解释，尤其是在靶向保守序列时，例如细菌 16S rRNA 区域。通过 Sigma′专有的 DNA 去除方法和严格的质量控制标准，我们可以确保产品中不存在最常见的污染性 DNA。每批MTP Taq均使用PCR和对细菌16S rRNA的保守区域、 Taq表达载体和人β-肌动蛋白基因有特异性的引物进行分析。
虽然 MTP Taq 确保以高质量、低污染的 DNA 聚合酶进行可靠的 PCR 扩增，但许多其它试剂可以将 DNA 污染物引入 PCR。为了进一步降低 PCR 过程中污染物 DNA 的风险，我们在每管 MTP TaqDNA聚合酶中加入了10× MTP Taq 缓冲液。每批10× MTP Taq缓冲液均经过与MTP Taq DNA 相同的严格质量控制测试，以确保没有可检出水平的污染性DNA。为防止假阳性 PCR 结果，在以MTP Taq DNA 聚合酶进行的 PCR 反应中只能使用不含 DNA 的试剂。
生化/生理作用
MTP™ Taq聚合酶可催化寡核苷酸引物驱动的、DNA模板依赖性地将dNTP掺入互补DNA链中。
特点和优势
- 低污染DNA聚合酶
- 防止由污染细菌DNA导致的假阳性PCR结果
组分
- MTP Taq DNA聚合酶(D7067)
- 10x MTP Taq缓冲液(M9943)
单位定义
一个酶活性单位是指在74 °C下，在30分钟内将10 nmol 脱氧核糖核苷三磷酸掺入酸性可沉淀DNA中所需的酶量。
其他说明
储存在-20°C。为方便起见，10x MTP Taq 缓冲液可以在室温下储存。
访问www.sigma-aldrich.com/mtptaq，查看有关MTP Taq DNA聚合酶的更多详细信息。
访问www.sigma-aldrich.com/specialtyenzymes，查看关于特色酶产品的更多信息。
法律信息
No license is conveyed with the purchase of this product under any of US Patents Nos. 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5′ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
MTP is a trademark of Sigma-Aldrich Co. LLC
Yi-Dong Wu et al.
Journal of clinical microbiology, 46(8), 2613-2619 (2008-06-14)
Sepsis is a serious disease with high mortality in newborns. It is very important to have a convenient and accurate method for pathogenic diagnosis of neonatal sepsis. We developed a method of simultaneous detection and Gram classification of clinically relevant
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PloS one, 15(3), e0230015-e0230015 (2020-03-20)
Obesity is a major health concern in many domesticated equids animals since it is related to metabolic abnormalities such as insulin dysregulation, hyperlipidaemia or laminitis. Ponies especially are known as "easy keepers" and are often affected by obesity and its
Li-Hua Chen et al.
Clinical pediatrics, 48(6), 641-647 (2009-05-02)
A method for the detection of bacterial pathogens in sepsis and bacterial meningitis with 16S rRNA gene- based real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) is developed. A total of 190 blood specimens and 5 cerebrospinal fluid specimens from neonates
Preventive Effect of Spontaneous Physical Activity on the Gut-Adipose Tissue in a Mouse Model That Mimics Crohn?s Disease Susceptibility
Maillard F, et al.
Cells, 8(1), 33-33 (2019)
C E Corless et al.
Journal of clinical microbiology, 38(5), 1747-1752 (2000-05-02)
A set of universal oligonucleotide primers specific for the conserved regions of the eubacterial 16S rRNA gene was designed for use with the real-time PCR Applied Biosystems 7700 (TaqMan) system. During the development of this PCR, problems were noted with
实验方案
MTP™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA.
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