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Merck
CN

D7304

Sigma-Aldrich

3,3′-二氨基联苯胺(DAB)液体底物系统 四盐酸盐

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UNSPSC代码:
12352203
NACRES:
NA.46

形式

liquid

质量水平

运输

wet ice

储存温度

2-8°C

一般描述

3,3′-二氨基联苯胺(DAB)是过氧化物酶的发色底物,可作免疫组织学和免疫印迹染色技术的染色剂。最终产品具有耐酒精性,因此DAB液体底物系统可以与多种复染剂和封片剂一起使用。DAB液体底物系统提供了产生快速、便捷的DAB底物溶液所需的所有色原体和缓冲液/过氧化物溶液。

应用

3,3′-二氨基联苯胺(DAB)四盐酸盐液体底物系统已被用作以下检测的底物:
  • 用于鉴定热休克蛋白(Hsp)在肝中表达变化的免疫印迹试验
  • 免疫染色后切片的可视化
  • 小鼠膝关节切片的免疫组织化学
  • 骆驼视前区kisseptin神经元的免疫标记
它与过氧化物酶反应产生的棕色沉淀可用于免疫组织学和免疫印迹染色过程。 不建议用于ELISA(微孔)检测。

注意

可在2-8℃下稳定保存至少一年。

数量

每个 DAB 液体底物系统均提供 250mL 的 DAB 底物溶液。

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

仅试剂盒组分

产品编号
说明

  • 10X DAB Liquid Chromogen 25 mL

  • ready-to-use buffer/peroxide solution 250 mL

警示用语:

Danger

危险分类

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Carc. 1B - Flam. Liq. 2 - STOT SE 1

靶器官

Eyes,Central nervous system

WGK

WGK 3

法规信息

危险化学品

分析证书(COA)

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J Balthazart et al.
Neuroscience, 153(4), 944-962 (2008-05-02)
Songbirds produce learned vocalizations that are controlled by a specialized network of neural structures, the song control system. Several nuclei in this song control system demonstrate a marked degree of adult seasonal plasticity. Nucleus volume varies seasonally based on changes
S R Pitkin et al.
Behavioural brain research, 119(2), 167-177 (2001-02-13)
The present study aimed to assess the role of advanced age in the development and manifestation of thiamine deficiency using an animal model of Wernicke-Korsakoff syndrome (WKS). Interactions between pyrithiamine-induced thiamine deficiency (PTD) and age were examined relative to working
Jennifer Walshe et al.
Developmental biology, 264(2), 522-536 (2003-12-04)
Characterisation of human craniofacial syndromes and studies in transgenic mice have demonstrated the requirement for Fgf signalling during morphogenesis of membrane bone of the cranium. Here, we report that Fgf activity is also required for development of the oro-pharyngeal skeleton
Adriana Martins Anciães et al.
Clinics (Sao Paulo, Brazil), 66(10), 1797-1803 (2011-10-21)
To verify the accordance of functional and morphometric parameters during the development of emphysema. BALB/c mice received a nasal drop of either papain or saline solution and were studied after 1, 3, 15, 28, and 40 days. Functional parameters, such
Ann Kakabadze et al.
Oncology letters, 13(3), 1811-1818 (2017-04-30)
The gold standard following segmental mandibulectomy is vascularized autologous bone graft in the form of the fibula flap. However, in bone reconstruction the use of autogenous bone does not always guarantee a successful outcome. The aim of the present investigation

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NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

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