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Merck
CN

D6815

Sigma-Aldrich

3,3′-二氨基联苯胺 (DAB) 增强型液体底物系统 四盐酸盐

peroxidase substrate, liquid, suitable for membrane applications

别名:

DAB membrane substrate

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About This Item

MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.83

产品名称

3,3′-二氨基联苯胺 (DAB) 增强型液体底物系统 四盐酸盐, for Membrane Applications

质量水平

表单

liquid

运输

wet ice

储存温度

2-8°C

SMILES字符串

Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H

InChI key

KJDSORYAHBAGPP-UHFFFAOYSA-N

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应用

DAB(3,3′-二氨基联苯胺四盐酸盐)双组分增强液底物系统是过氧化物酶的沉淀底物系统。DAB 双组分液体底物系统会产生棕色沉淀,可用于免疫组织学和免疫印迹染色程序。
3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride has been used as a substrate for peroxidase coupled secondary antibody.
  • in paraffin heart sections of mice for immunohistochemical and immunofluorescent staining
  • in immunotransfer of salivary protein of laboratory Lutzomyia ovallesi
  • in immunoperoxidase staining of lung tissue

生化/生理作用

3,3′-Diaminobenzidine (DAB) is a chromogenic substrate for peroxidase enzyme. It is converted to an insoluble precipitate after oxidation by peroxidase. This feature is exploited for visualization in histochemical (HC) and immunohistochemical (IHC) staining.

包装

Set consists of 4 x 100 ml Solution A and 15 ml Solution B.

注意

在 2-8℃ 下储存于原容器中时可稳定储存长达 18 个月。

数量

Each DAB Two-component Liquid Substrate System will provide 500 mL of the DAB substrate solution.

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • 3, 3′-Diaminobenzidine enhanced liquid substrate system 4 x 100

象形图

Health hazard

警示用语:

Danger

危险声明

危险分类

Carc. 1B - Muta. 2

储存分类代码

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

法规信息

监管及禁止进口产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Preclinical efficacy studies of influenza A haemagglutinin precursor cleavage loop peptides as a potential vaccine
Miller DS, et al.
The Journal of General Virology, 92, 1152-1161 (2011)
Władysław Polcyn
Acta biochimica Polonica, 55(4), 761-766 (2008-11-19)
Previously, we showed that anaerobic induction of respiratory nitrate reductase (NR) activity in Bradyrhizobium sp. (Lupinus) USDA 3045 is strongly enhanced by nitrate or nitrite through de novo synthesis. Here, multiple NR-active soluble forms, ranging from 75 kDa to 190
Preclinical efficacy studies of influenza A haemagglutinin precursor cleavage loop peptides as a potential vaccine
Miller DS, et al.
The Journal of General Virology, 92(5), 1152-1161 (2011)
Acute erythropoietin cardioprotection is mediated by endothelial response
Teng R, et al.
Basic Research in Cardiology, 106, 343-354 (2011)
Nanette Mittereder et al.
Journal of immunology (Baltimore, Md. : 1950), 197(2), 491-503 (2016-06-15)
ICOS, a member of the CD28 family, represents a key molecule that regulates adaptive responses to foreign Ags. ICOS is prominently expressed on T follicular helper (TFH) cells, a specialized CD4(+) T cell subset that orchestrates B cell differentiation within

商品

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

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