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Merck
CN

D6046

Sigma-Aldrich

DMEM - low glucose

With sodium bicarbonate, sodium pyruvate and ʟ-glutamine, sterile liquid, suitable for cell culture

别名:

DME, DMEM

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About This Item

MDL编号:
UNSPSC代码:
12352207
NACRES:
NA.75

product name

杜氏改良 Eagle 培养基-低葡萄糖, With 1000 mg/L glucose, L-glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

质量水平

无菌性

sterile-filtered

形式

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

L-glutamine: yes
sodium pyruvate: yes
phenol red: yes
NaHCO3: yes
HEPES: no
glucose: low

运输

ambient

储存温度

2-8°C

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一般描述

该DMEM低葡萄糖培养基是一种含丙酮酸钠的1x完全培养基。 它也不同于原始DMEM-Hi配方,后者以吡哆醇代替吡哆醛。 吡哆醛是一种不稳定的培养基成分。

应用

Dulbecco改良Eagle培养基-中低葡萄糖型是原始Dulbecco配方的完全补充液体形式,但有一个重要区别。使用吡哆醇取代了吡哆醛。这种改良已被证明可以提高培养基的稳定性。
Dulbecco改良Eagle培养基-低葡萄糖型已被用于哺乳动物细胞的维持/培养。

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

分析证书(COA)

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Huong Thi Pham et al.
PLoS genetics, 14(8), e1007574-e1007574 (2018-08-04)
The broadly conserved bacterial signalling molecule cyclic-di-adenosine monophosphate (c-di-AMP) controls osmoresistance via its regulation of potassium (K+) and compatible solute uptake. High levels of c-di-AMP resulting from inactivation of c-di-AMP phosphodiesterase activity leads to poor growth of bacteria under high
Nicotine increases cancer stem cell population in MCF-7 cells.
Hirata N et al.
Biochemical and Biophysical Research Communications, 403, 138-138 (2010)
Nuclear receptor liver X receptor is O-GlcNAc-modified in response to glucose.
Anthonisen EH et al.
The Journal of Biological Chemistry, 285, 1607-1607 (2010)
June Guo et al.
Metabolism: clinical and experimental, 68, 108-118 (2017-02-12)
Our laboratory has shown that insulin's effect to decrease neointimal thickness after arterial injury is greatly diminished in insulin resistant conditions. Thus, in these conditions, a better alternative to insulin could be to use an insulin sensitizing agent. Metformin, the
High glucose-induced mitochondrial respiration and reactive oxygen species in mouse cerebral pericytes is reversed by pharmacological inhibition of mitochondrial carbonic anhydrases: Implications for cerebral microvascular disease in diabetes.
Shah GN et al.
Biochemical and Biophysical Research Communications, 440, 354-354 (2013)

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