D5921
DMEM - low glucose
With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture
别名:
DME, DMEM
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产品名称
杜氏改良 Eagle 培养基-低葡萄糖, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
phenol red: no
sodium pyruvate: no
glucose: low
HEPES: no
L-glutamine: no
NaHCO3: yes
shipped in
ambient
storage temp.
2-8°C
General description
杜氏改良Eagle培养基（DMEM）是对Eagle基础培养基 (BME)的改良，含有四倍浓度的氨基酸和维生素以及其他补充组分。据报告首个原始DME配方已用于培养含有1,000 mg/L葡萄糖的胚胎小鼠细胞。4,500 mg/L葡萄糖的修饰对培养某些类型的细胞效果最佳。已证明DME可提高培养基的稳定性。
Application
杜氏改良Eagle低葡萄糖培养基已用于培养：
- 结肠癌SW620活细胞
- 人乳腺癌细胞系MCF-7(13)、KPL和MDA-MB-231
- 哺乳动物和犬源性脂肪源性间充质干细胞
Preparation Note
补充 0.584 g/L L -谷氨酰胺。
Other Notes
使用干细胞或细胞克隆的研究者通常更喜欢不含酚红的培养基。
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Fabian E Ortega et al.
eLife, 8 (2019-02-06)
Listeria monocytogenes hijacks host actin to promote its intracellular motility and intercellular spread. While L. monocytogenes virulence hinges on cell-to-cell spread, little is known about the dynamics of bacterial spread in epithelia at a population level. Here, we use live
Kenji Ohe et al.
The Journal of steroid biochemistry and molecular biology, 182, 21-26 (2018-04-22)
The high-mobility group A protein 1a (HMGA1a) protein is known as an oncogene whose expression level in cancer tissue correlates with the malignant potential, and known as a component of senescence-related structures connecting it to tumor suppressor networks in fibroblasts.
Tao Dong et al.
Molecular human reproduction, 26(6), 441-451 (2020-04-26)
Concentrations of 2-methoxyestradiol (2ME2), a principal metabolite of estradiol, are significantly lower in women with severe preeclampsia. Nitric oxide (NO) released by endothelial nitric oxide synthase (eNOS) plays an important role in regulating cardiovascular homeostasis. Importantly, high-density lipoprotein (HDL) stimulates
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| D5921-500ML | 04061833587065 |
| D5921-6X500ML | 04061833587072 |