D5694
Anti-DCP1A (N-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
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别名:
Anti-DCP1 Decapping enzyme 1, homolog A, Anti-SMAD 4-interacting transciption factor, Anti-SMAD 4-interacting transciptional co-activator, Anti-SMAD4IP1, Anti-SMIF
UNSPSC代码:
12352203
NACRES:
NA.41
生物来源
rabbit
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
分子量
antigen ~70 kDa
种属反应性
rat, human, mouse
浓度
~1.0 mg/mL
技术
immunoprecipitation (IP): 5-10 μg using lysates of HEK-293T
indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde-fixed NIH-3T3 cells over-expressign human DCP1A
western blot: 1-2 μg/mL using lysates of HEK-293T cells over-expressing human DCP1A
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... DCP1A(55802)
mouse ... Dcp1a(75901)
rat ... Dcp1a(361109)
一般描述
Dcp1 colocalizes with Dcp2 in distinct cytoplasmic foci along with other proteins involved in the 5′ to 3′ mRNA decay. These foci are termed PB (processing bodies) or DCP-bodies. DCP1A and DCP1B are the two distinct genes of human DCP1. They share ~70% homology in their N-termini and ~30% homology in their full length.
Human cells consists of two decapping protein 1a (DCP1a) homologues, hDcp1a and hDcp1b, that are coded by two distinct genes.
应用
Anti-DCP1A (N-terminal) antibody produced in rabbit has been used in:
- immunoblotting
- immunofluorescence
- immunoprecipitation
生化/生理作用
Dcp1 cleaves the m7G mRNA cap in the 5′ to 3′ mRNA decay pathway. Decapping is a critical and highly regulated step in the turnover of mRNA which involves decapping enzymes that hydrolyze the cap structure at the 5′ mRNA.
Human decapping protein 1a (hDCP1a) acts as a processing body (PB) marker.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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价格
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
新产品
Adva Aizer et al.
PloS one, 8(1), e49783-e49783 (2013-01-10)
Processing bodies (PBs) are non-membranous cytoplasmic structures found in all eukaryotes. Many of their components such as the Dcp1 and Dcp2 proteins are highly conserved. Using live-cell imaging we found that PB structures disassembled as cells prepared for cell division
Martin Fenger-Grøn et al.
Molecular cell, 20(6), 905-915 (2005-12-21)
Decapping is a key step in mRNA turnover. However, the composition and regulation of the human decapping complex is poorly understood. Here, we identify three proteins that exist in complex with the decapping enzyme subunits hDcp2 and hDcp1: hEdc3, Rck/p54
Jens Lykke-Andersen
Molecular and cellular biology, 22(23), 8114-8121 (2002-11-06)
Decapping is a key step in general and regulated mRNA decay. In Saccharomyces cerevisiae it constitutes a rate-limiting step in the nonsense-mediated decay pathway that rids cells of mRNAs containing premature termination codons. Here two human decapping enzymes are identified
Christy Fillman et al.
Current opinion in cell biology, 17(3), 326-331 (2005-05-20)
Decapping is a central step in eukaryotic mRNA turnover. Recent studies have identified several factors involved in catalysis and regulation of decapping. These include the following: an mRNA decapping complex containing the proteins Dcp1 and Dcp2; a nucleolar decapping enzyme
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