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安全信息

D5637

Sigma-Aldrich

3,3-二氨基联苯胺 四盐酸盐 水合物

≥96%

别名:

(1,1′-联苯)-3,3′,4,4′-四胺四盐酸盐二水合物

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About This Item

线性分子式:
C12H14N4 · 4HCl · xH2O
CAS号:
868272-85-9
EC 号:
231-018-9
MDL编号:
MFCD08273058
UNSPSC代码:
12171500
PubChem化学物质编号:
57654109
NACRES:
NA.47

质量水平

200

检测方案

≥96%

形式

powder, crystals or chunks

技术

titration: 96-104% (with NaOH)

颜色

white to light brown, and Faint Red and Faint Grey to Light Grey

mp

280 °C

溶解性

H2O: 50 mg/mL

应用

diagnostic assay manufacturing
hematology
histology

储存温度

−20°C

SMILES字符串

O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4.4ClH.H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;;/h1-6H,13-16H2;4*1H;1H2

InChI key

DXWSCXIZIHILNP-UHFFFAOYSA-N

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相关类别

一般描述

3,3′-二氨基联苯胺四盐酸盐水合物是一种常用于免疫组织化学和其他染色技术的显色剂，可在生物样品中可视化抗原或抗体的存在。DAB HCl 设计用作免疫组织学染料，以可视化观测细胞中的过氧化物酶活性。可通过分光光度计测量DAB氧化情况。

应用

3,3′-二氨基联苯胺四盐酸盐水合物适用于免疫组织学染色方案、蛋白质印迹和 FISH（荧光原位杂交）。DAB 用于研究早期白内障时的上皮细胞凋亡，以及常染色体显性遗传性多囊肾 (PKD) 的免疫组织化学方案。

生化/生理作用

在过氧化物存在的条件下，DAB用作过氧化物酶反应中的氢供体。氧化后的DAB形成棕色不溶性复合物。

象形图

Health hazardExclamation mark
GHS08,GHS07

警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

危险化学品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. What is Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB), soluble in?

    DAB HCl is soluble in water at 50 mg/ml yielding a clear to slightly hazy solution. For consistency, it is important to allow DAB to come to room temperature before making solutions. Water quality is extremely important, so only high quality deionized or distilled water should be used to make DAB solutions.

  2. What is the solution stability of Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB)?

    In our experience, solutions of DAB should be made immediately prior to use for best results. If and only if necessary, solutions can be kept in the dark and refrigerated for overnight storage. Based on our Immunology Department’s experience, storing the solution overnight did not give good reproducibility. However, the literature indicates that it is possible to store solutions of DAB at -20°C. DAB dissolved in Tris buffered saline (pH 7.6) at 0.6 mg/ml was reported to be stable for several weeks at 4°C.

  3. Can the solutions of Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB), be filtered?

    DAB solution may be filtered through a 0.2 micron filter immediately prior to use.

  4. Do you have a staining protocol for Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB)?

    Dissolve 10 mg in 15 mL of Tris buffered saline, pH 7.6 (Tris-buffered saline tablet, Sigma Product No. T5030 may be used). Add 12 uL of fresh 30% hydrogen peroxide (Sigma Product No. H1009) prior to use. Solution may be filtered through a 0.2 micron filter immediately prior to use if necessary. DAB and hydrogen peroxide concentrations may be adjusted to suit individual applications. The stain can be enhanced with Ni. Make up 0.3% (w/v) NiCl2 in water. Add 1 mL of the NiCl2 stock to 9 mL DAB solution.

  5. What is the shelf life of Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB)?

    This product has a shelf life of 3 years.

  6. How is Product D5637, 3,3´-Diaminobenzidine tetrahydrochloride hydrate (DAB), shipped and stored?

    The product is shipped at ambient temperature. However, it is stored at -20 degrees C for long term storage.

  7. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  8. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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The Journal of Biological Chemistry, 271, 611-614 (1996)
A new sensitive colorimetric assay for peroxidase using 3,3'-diaminobenzidine as hydrogen donor.
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Analytical Biochemistry, 55, 554-562 (1973)
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