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product name
杜氏改良 Eagle 培养基-低葡萄糖, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
质量水平
无菌性
sterile-filtered
形式
liquid
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
组分
NaHCO3: yes
phenol red: yes
glucose: low
HEPES: no
sodium pyruvate: yes
L-glutamine: no
运输
ambient
储存温度
2-8°C
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一般描述
Dulbecco′s改良Eagle培养基(DMEM)是Eagle基础培养基(BME)的改良版，含有更高浓度的氨基酸和维生素及其他添加成分。据报道，原本的DMEM配方开发用于小鼠胚胎细胞培养，含有1,000 mg/L葡萄糖。改为4,500 mg/L葡萄糖后，适合特定类型的细胞生长。这种改良已被证明能够改善培养基的稳定性。
应用
Dulbecco′s改良Eagle′s培养基（低糖）已用于培养：
- 人骨肉瘤细胞和HeLa细胞
- 原代人间充质干细胞(hBMSCs)
- 人胚肾细胞系
重悬
补充0.584 g/L谷氨酰胺。
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产品编号
说明
价格
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
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Scott A Hinger et al.
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The regulation and functional roles of secreted coding and long noncoding RNAs (lncRNAs; >200 nt) are largely unknown. We previously showed that mutant KRAS colorectal cancer (CRC) cells release extracellular vesicles (EVs) containing distinct proteomes, microRNAs (miRNAs), and circular RNAs.
William A Lackington et al.
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