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Merck
CN

D5546

Sigma-Aldrich

DMEM - low glucose

With sodium bicarbonate and sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

别名:

DME, DMEM

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About This Item

MDL编号:
UNSPSC代码:
12352207
NACRES:
NA.75

产品名称

杜氏改良 Eagle 培养基-低葡萄糖, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

质量水平

无菌性

sterile-filtered

表单

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

NaHCO3: yes
phenol red: yes
glucose: low
HEPES: no
sodium pyruvate: yes
L-glutamine: no

运输

ambient

储存温度

2-8°C

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一般描述

Dulbecco′s改良Eagle培养基(DMEM)是Eagle基础培养基(BME)的改良版,含有更高浓度的氨基酸和维生素及其他添加成分。据报道,原本的DMEM配方开发用于小鼠胚胎细胞培养,含有1,000 mg/L葡萄糖。改为4,500 mg/L葡萄糖后,适合特定类型的细胞生长。这种改良已被证明能够改善培养基的稳定性。 

应用

Dulbecco′s改良Eagle′s培养基(低糖)已用于培养:

  • 人骨肉瘤细胞和HeLa细胞
  • 原代人间充质干细胞(hBMSCs)
  • 人胚肾细胞系

重悬

补充0.584 g/L谷氨酰胺。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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