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安全信息

D4818

Sigma-Aldrich

分散酶®I

protease

别名:

蛋白酶 来源于多粘芽孢杆菌

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About This Item

CAS号:
42613-33-2
EC 号:
255-914-4
MDL编号:
MFCD02253017
UNSPSC代码:
12352204
NACRES:
NA.54

质量水平

200

表单

lyophilized solid

比活

≥10 unit/mg solid

技术

cell culture | mammalian: suitable
single cell analysis: suitable

储存温度

2-8°C

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应用

分散酶I已被用于肺组织消化和流式染色处理，以及小鼠CD4细胞分离。该酶还被用于消化切割伤口及少量周围皮肤，用于GFP+（绿色荧光蛋白）细胞检测。本研究旨在探讨骨髓间充质干细胞的分化和血管生成对创面愈合的影响。它还被用于在小鼠真皮成纤维细胞分离过程中去除表皮。

适用于制备测序用单细胞悬液。
分散酶I已被用于评估羊膜在愈合过程早期阶段对伤口大小的影响。 分散酶I还被用于研究以二酪氨酸为底物的蛋白酶测定。

生化/生理作用

分散酶I是一种快速、有效、温和的中性蛋白酶，可以将完整的表皮层与真皮层分开。它还可以将培养物中的完整上皮细胞层与下层分离。该酶可在消化基底膜区域的同时保留上皮细胞的活力。它还可用于防止悬浮培养物中的细胞结块。该蛋白酶可水解纤连蛋白和IV型胶原蛋白，但不能水解层粘连蛋白、V型胶原蛋白、血清白蛋白或转铁蛋白。它可以水解非极性氨基酸残基的N-末端肽键。它优先水解具有暴露疏水氨基酸残基的变性和细胞间蛋白质。Ca2+, Mg2+, Mn2+, Fe2+, Fe3+ 和Al3+可以激活该酶活性。EDTA、EGTA、Hg2+ 和其他重金属可抑制该酶活性。每g-mol纯化分散酶I含有1g锌原子。如果通过EDTA或EGTA等螯合剂除去锌成分，则产生无活性的酶蛋白。该酶不受血清抑制。

单位定义

若无其他说明，则一个酶活性单位是指在pH7.5、37℃条件下每分钟水解酪蛋白并产生相当于1.0 μM (181 μg) 酪氨酸的颜色（使用Folin-Ciocalteu试剂显色）所需的酶量。

外形

冻干粉末，含醋酸钙

法律信息

Dispase is a registered trademark of Godo Shusei Co., Ltd.

象形图

Health hazardExclamation mark
GHS08,GHS07

警示用语：

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
0000377318
0000358196
0000344215
0000331835
0000316870

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. At what concentration should Product D4818, Dispase, be used?

    To dissociate cells from ECM (extracellular matrix) use at a concentration of 0.6 - 2.4 units/ml.

  4. What is the activity of the Product D4818, Dispase, which I received?

    Each lot will have a specific activity that can be found on our website on the certificate of analysis.

  5. What is the difference between Product D4693, Dispase II  and Product D4818, Dispase I?

    Product D4693 is a lyophilized powder containing calcium acetate and milk sugar as a stabilizer. The activity specification is >0.5 unit/mg solid. Product D4818 does not contain any milk sugar and has an activity specification of >10 units/mg solid.

  6. What is the solubility of Product D4818, Dispase I?

    The enzyme may be reconstituted at 1 mg/ml in 10 mM sodium acetate buffer, pH 7.5 with 5 mM calcium acetate.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Yaojiong Wu et al.
Stem cells (Dayton, Ohio), 25(10), 2648-2659 (2007-07-07)
Although chronic wounds are common, treatment for these disabling conditions remains limited and largely ineffective. In this study, we examined the benefit of bone marrow-derived mesenchymal stem cells (BM-MSCs) in wound healing. Using an excisional wound splinting model, we showed
Alizée Vercauteren Drubbel et al.
Cell stem cell, 28(8), 1411-1427 (2021-04-22)
Columnar metaplasia of the esophagus is the main risk factor for esophageal adenocarcinoma. There is a lack of evidence to demonstrate that esophageal progenitors can be the source of columnar metaplasia. In this study, using transgenic mouse models, lineage tracing
Alessandra Sacco et al.
Nature, 456(7221), 502-506 (2008-09-23)
Adult muscle satellite cells have a principal role in postnatal skeletal muscle growth and regeneration. Satellite cells reside as quiescent cells underneath the basal lamina that surrounds muscle fibres and respond to damage by giving rise to transient amplifying cells
Liwen Chen et al.
PloS one, 4(9), e7119-e7119 (2009-09-23)
Studies have shown that allogeneic (allo-) bone marrow derived mesenchymal stem cells (BM-MSCs) may enhance tissue repair/regeneration. However, recent studies suggest that immune rejection may occur to allo-MSCs leading to reduced engraftment. In this study, we compared allo-BM-MSCs with syngeneic
Xie Yingjun et al.
Stem cell research, 41, 101583-101583 (2019-11-08)
Asparagine synthetase (ASNS) deficiency (ASNSD; MIM #615574) is a rare neurodevelopmental disorder caused by mutations in the ASNS gene. The ASNS gene maps to cytogenetic band 7q21.3 and is 35 kb long. ASNSD is characterised by congenital microcephaly, severely delayed psychomotor
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