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Merck
CN

D4168

Sigma-Aldrich

SIGMAFAST 3,3′-二氨基联苯胺片

tablet, To prepare 1 mL

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CAS号:
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.83

质量水平

形式

tablet

溶解性

deionized water: 1 mL, clear, pink

储存温度

−20°C

SMILES字符串

Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2

InChI key

HSTOKWSFWGCZMH-UHFFFAOYSA-N

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应用

开发用于免疫组织学和免疫印迹,作为过氧化物酶活性定位的沉淀底物。DAB 是免疫组织学的首选底物,因为其可产生对酒精具有抗性的强烈棕黑色染料。用 DAB 染色的载玻片可脱水,封片于树脂介质中,并储存以备将来引用。SIGMAFAST DAB 片剂不需要额外的缓冲液或步骤来制备活性底物溶液。片剂单独包装在铝箔包装中

重悬

将每片片剂溶于 1 mL 去离子水中,得到含 DAB 和过氧化氢尿素的即用型缓冲液。

法律信息

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

警示用语:

Danger

危险分类

Carc. 1B - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Ox. Liq. 3 - Skin Irrit. 2 - STOT SE 3

靶器官

Central nervous system

WGK

WGK 3

闪点(°F)

53.6 °F

闪点(°C)

12 °C

法规信息

危险化学品

分析证书(COA)

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Kristin M Byers et al.
ACS omega, 5(9), 4673-4681 (2020-03-17)
Two-dimensional paper networks (2DPNs) have enabled the use of paper-based platforms to perform multistep immunoassays for detection of pathogenic diseases at the point-of-care. To date, however, detection has required the user to provide multiple signal enhancement solutions and been limited
D Randazzo et al.
American journal of physiology. Cell physiology, 312(1), C16-C28 (2016-10-28)
We recently reported that skeletal muscle fibers of obscurin knockout (KO) mice present altered distribution of ankyrin B (ankB), disorganization of the subsarcolemmal microtubules, and reduced localization of dystrophin at costameres. In addition, these mice have impaired running endurance and
Nour Ershaid et al.
Nature communications, 10(1), 4375-4375 (2019-09-29)
Cancer-Associated Fibroblasts (CAFs) were shown to orchestrate tumour-promoting inflammation in multiple malignancies, including breast cancer. However, the molecular pathways that govern the inflammatory role of CAFs are poorly characterised. In this study we found that fibroblasts sense damage-associated molecular patterns
Itamar Goren et al.
Nitric oxide : biology and chemistry, 87, 31-42 (2019-03-14)
The gaseous mediators nitric oxide (NO), carbon monoxide (CO) and lately also hydrogen sulfide (H2S) have been described to contribute to the interplay of protein type- and lipid mediators in the regulation of wound healing. In particular, the recently reported
Dileyra Adiguzel et al.
PloS one, 14(5), e0216814-e0216814 (2019-05-24)
Recent studies indicate that FoxO1 has roles in female reproductive system, especially in maternal endometrium. Although various cellular aspects and molecular pathways have been identified, the exact molecular characteristics of embryo implantation are still not completely understood. In this study

商品

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

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