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Merck
CN

D3514

Sigma-Aldrich

4,4'-二异硫氰酸十八烯-2,2'-二磺酸 二钠盐 水合物

≥80% (elemental analysis), powder

别名:

DIDS, 二钠4,4 & # 8242;-二异硫氰酸芪-2,2 & # 8242;-二磺酸酯

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About This Item

经验公式(希尔记法):
C16H8N2Na2O6S4 · xH2O
分子量:
498.48 (anhydrous basis)
Beilstein:
8179433
MDL编号:
UNSPSC代码:
12352200
PubChem化学物质编号:
NACRES:
NA.25

检测方案

≥80% (elemental analysis)

形式

powder

反应适用性

reagent type: cross-linking reagent

颜色

yellow

溶解性

0.1 M potassium bicarbonate: 50 mg/mL

储存温度

2-8°C

SMILES字符串

[Na+].[Na+].[O-]S(=O)(=O)c1cc(ccc1\C=C\c2ccc(cc2S([O-])(=O)=O)N=C=S)N=C=S

InChI

1S/C16H10N2O6S4.2Na/c19-27(20,21)15-7-13(17-9-25)5-3-11(15)1-2-12-4-6-14(18-10-26)8-16(12)28(22,23)24;;/h1-8H,(H,19,20,21)(H,22,23,24);;/q;2*+1/p-2/b2-1+;;

InChI key

GEPAYBXVXXBSKP-SEPHDYHBSA-L

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应用

使用4,4′-二异硫氰基二苯乙烯-2,2′-二磺酸二钠盐水合物:
  • 兔带3蛋白抑制剂(19)
  • 抑制大脑切片组织(20)中碳酸氢盐转运体
  • 作为胚胎(21)中HCO3-/Cl-交换剂(阴离子交换剂)

一种带负电荷的同双功能交联剂。异硫氰酸酯基团在pH 9.0-10.0下与伯胺反应。

生化/生理作用

4,4′-二异硫氰基二苯乙烯-2,2′-二磺酸是一种含异硫氰酸酯残基的阴离子烷化剂。它是一种带负电荷的同双功能交联试剂。4,4′-二异硫氰基二苯乙烯-2,2′-二磺酸是一种用于细胞转运研究的细胞阴离子渗透性特异性抑制剂。异硫氰酸酯基团在pH 9.0-10.0下与伯胺反应。4,4′-二异硫氰基二苯乙烯-2,2′-二磺酸烷基化赖氨酸残基,并通过基于靶向膜的负离子转运体间接抑制细胞凋亡。此外,还能抑制钙在小囊泡中的转运。
一种用于细胞转运研究的细胞阴离子渗透性特异性抑制剂。

象形图

Health hazardExclamation mark

警示用语:

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


分析证书(COA)

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  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. What is the solubility of 4,4´-Diisothiocyanatostilbene-2,2´-disulfonic acid disodium salt (Product No. D3514)?

    Product No. D3514 is soluble in 0.1 M KHCO3 (50 mg/mL) yielding a hazy, yellow-green solution. This may require heat for complete solubilization. DIDS is also soluble in DMSO.

  7. What is the solution stability of 4,4´-Diisothiocyanatostilbene-2,2´-disulfonic acid disodium salt (Product No. D3514)?

    We have no specific solution stability data for Product No. D3514, however, isothiocyanates are unstable in water and should not be stored in aqueous solutions.

  8. What are the fluorescent properties of 4,4´-Diisothiocyanatostilbene-2,2´-disulfonic acid disodium salt (Product No. D3514)?

    Product No. D3514 has an excitation maximum at a wavelength of 342 nm, and an emission maximum at a wavelength of 418 nm, measured in water.

  9. Is there a reference for the use of 4,4´-Diisothiocyanatostilbene-2,2´-disulfonic acid disodium salt (Product No. D3514) as a cross-linking reagent?

    The spectral properties of Product No. D3514 are: ?max: 220 nm, 265 nmExtinction coefficient: EmM = 3000 (280 nm, water), EmM = 54 (342 nm, water).  This information is from J. Membrane Biol., 29, 147-177 (1976).  

  10. Is there a reference for the use of 4,4´-Diisothiocyanatostilbene-2,2´-disulfonic acid disodium salt (Product No. D3514) as a cross-linking reagent?

    Product No. D3514 binds covalently (irreversibly) to the outer surface of human erythrocyte membrane protein, functioning as an anion transport inhibitor. It also can be used to cross-link membrane anion transport sites.  A good cross-linking reference is J. Membrane Biol., 29, 147-177 (1976).

  11. My question is not addressed here, how can I contact Technical Service for assistance?

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