Other Notes
Sigma′s Lambda DNA Mixed Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
For optimal resolution, the recommended agarose gel concentration is 0.4%.
Application
Lambda DNA Mixed Digest has been used for DNA electrophoresis.
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
General description
Sigma′s Lambda DNA Mixed Digest contains 10 fragments from 1,503 − 48,502 bp. Six ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Preparation Note
Bacteriophage lambda DNA was completely digested with by Apa I, Kpn I, Xba I and Xho I in separate reactions, sticky ends filled with DNA polymerase I Klenow (to prevent reannealing), mixed with uncut bacteriophage lambda DNA, and then purified by phenol extraction.
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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A rapid (100 min) method for isolating high yield and quality DNA from leaves, roots and coleoptile of wheat (Triticum aestivum L.) suitable for apoptotic and other molecular studie
Hameed
International Journal of Agriculture and Biology, 383-387 (2004)
商品
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.
选择适合的标记物和分子量标准，用于测定电泳分离样品的核酸大小。通过琼脂糖或聚丙烯酰胺凝胶检测DNA、RNA和PCR生成片段的大小。
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Datasheet
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