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Merck
CN

D2189

Recombinant CRM197 expressed in Pseudomonas fluorescens, [Glu52]- Diphtheria toxin

lyophilized powder

别名:

[Glu52]-Diphtheria toxin from Corynebacterium diphtheriae, CRM 197

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化学文摘社编号:
92092-36-9
UNSPSC Code:
12352202

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form

lyophilized powder

storage temp.

2-8°C

Quality Level

200

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Packaging

Package size based on protein content

Physical form

Lyophilized powder containing sodium phosphate, sucrose and polysorbate 80

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

新产品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLBW3541
SLBV9634
SLBV5814
SLBT2959
SLBT0771

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I reconstitute Product D2189, [Glu52]-Diphtheria toxin from Corynebacterium diphtheriae?

    The vial can be reconstituted with 1.0 mL of sterile water. Each vial contains 1 mg of Diphtheria toxin CRM mutant, sodium phosphate buffer, and lactose. It can sometimes be hard to get the product into solution due to the lactose used as a stabilizer. The supplier suggests slight warming (to at least room temperature or 30 °C, but not more than 37 °C) followed by a brief vortex to break up the filaments.   

  4. What kind of mutant, is Product D2189, [Glu52]-Diphtheria toxin from Corynebacterium diphtheriae, and how is this product different from Product No. D0564?

    Product No. D0564 is the wild-type toxin and possesses enzymatic activity.  The Glu52 diptheria toxin, also known as CRM197, has a mutation in fragment A (a glycine to glutamic acid substitution at position 52) that results in the complete loss of enzymatic activity. More information on the mutant can be found in the following reference: Uchida, T., Pappenheimer, Jr., A.M. and Harper, A.A.,  J. Biol. Chem., 248, 3851-3854 (1973).

  5. What is the molecular weight of Product D2189, [Glu52]-Diphtheria toxin from Corynebacterium diphtheriae?

    On reducing SDS gels, this protein migrates as a single band of an approximate molecular weight of 63,000 Daltons.      

  6. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Fabio Fiorino et al.
Vaccine, 30(43), 6111-6114 (2012-06-19)
Typhoid fever is a public health problem, especially among young children in developing countries. To address this need, a glycoconjugate vaccine Vi-CRM₁₉₇, composed of the polysaccharide antigen Vi covalently conjugated to the non-toxic mutant of diphtheria toxin CRM₁₉₇, is under
Enrico Malito et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(14), 5229-5234 (2012-03-21)
CRM197 is an enzymatically inactive and nontoxic form of diphtheria toxin that contains a single amino acid substitution (G52E). Being naturally nontoxic, CRM197 is an ideal carrier protein for conjugate vaccines against encapsulated bacteria and is currently used to vaccinate
F Micoli et al.
Vaccine, 30(5), 853-861 (2011-12-17)
A conjugate vaccine for Salmonella enterica serovar Typhi was produced by chemically linking Vi, purified from Citrobacter, to the non-toxic mutant diphtheria toxin CRM(197) via an adipic dihydrazide spacer using N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide coupling chemistry. The polysaccharide purification process was developed based
Naoko Kunami et al.
Anticancer research, 31(7), 2483-2488 (2011-08-30)
The therapeutic outcome for T-cell acute lymphoblastic leukemia (T-ALL) remains poor; thus, novel, targeted therapies are urgently needed. Recently, we showed that heparin-binding epidermal growth factor-like growth factor (HB-EGF), a member of the EGF family, is a promising target for
Geert J Schenk et al.
Journal of controlled release : official journal of the Controlled Release Society, 158(1), 139-147 (2011-10-11)
Efficient delivery of drugs to specific cellular reservoirs is of particular importance for therapeutics that are not able to pass cellular barriers and that may have unwanted side effects in off-target tissues. Heparin-binding epidermal growth factor (HB-EGF) is expressed on
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