一般描述
Diffinity RapidTip可有效去除dNTP、引物和引物二聚体，并同时为从100 bp至10 kb纯的DNA片段提供超过90％的回收率。该功能性移液器吸头包含PCR纯化所需的一切。通过专有的吸附技术，该吸头可对PCR组分具有不同的亲和力。所分配的溶液可产生能力够即用于如DNA测序、SNP分析和微阵列打印等下游应用的高质量纯化DNA。
应用
Diffinity RapidTip®已被用于PCR产物纯化。
特点和优势
- 单步
- 回收90%的高质量dsDNA
- 优化用于25 μL RXN
法律信息
RapidTip is a registered trademark of Diffinity Genomics, Inc.
H Kühlwein et al.
Journal of applied microbiology, 115(5), 1091-1106 (2013-07-31)
To assess the effects of dietary Saccharomyces cerevisiae β-(1,3)(1,6)-D-glucan supplementation (MacroGard(®)) on mirror carp (Cyprinus carpio L.) intestinal microbiota and ultrastructure of the enterocyte apical brush border. Carp were fed either a control diet or diets supplemented with 0.1, 1
Sarah L Dean et al.
Molecular ecology, 23(6), 1364-1378 (2013-10-12)
Nitrogen (N) deposition rates are increasing globally due to anthropogenic activities. Plant community responses to N are often attributed to altered competitive interactions between plants, but may also be a result of microbial responses to N, particularly root-associated fungi (RAF)
Teruaki Nakatsuji et al.
Nature communications, 4, 1431-1431 (2013-02-07)
Commensal microbes on the skin surface influence the behaviour of cells below the epidermis. We hypothesized that bacteria or their products exist below the surface epithelium and thus permit physical interaction between microbes and dermal cells. Here to test this
Michael J Goblirsch et al.
PloS one, 8(7), e69831-e69831 (2013-07-31)
A major hindrance to the study of honey bee pathogens or the effects of pesticides and nutritional deficiencies is the lack of controlled in vitro culture systems comprised of honey bee cells. Such systems are important to determine the impact
C N Roterman et al.
Proceedings. Biological sciences, 280(1764), 20130718-20130718 (2013-06-21)
The phylogeny of the superfamily Chirostyloidea (Decapoda: Anomura) has been poorly understood owing to limited taxon sampling and discordance between different genes. We present a nine-gene dataset across 15 chirostyloids, including all known yeti crabs (Kiwaidae), to improve the resolution
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