产品名称
脱氧核糖核酸 钠盐 来源于小牛胸腺, Type I, fibers
type
Type I
form
fibers
color
white
storage temp.
2-8°C
Quality Level
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Preparation Note
该产物采用可引起剪切的方法进行提取，产生双链和单链DNA的高度聚合混合物。 但是，双链DNA是主要形式。
DNA溶液已在4℃，pH 7.5-8，含1mM EDTA且不含抑菌剂的10mM Tris中成功保存数月。 在低浓度（μg/ml）条件下，DNA倾向于吸附在塑料管表面上。不建议将DNA储存在高碱性溶液中，因为DNA在pH大于8.0的碱性条件下会降解。
本品能够以2 mg/ml的浓度溶于水。为了进一步减少DNA剪切，不应使用超声处理或搅拌。 建议在0-4°C轻轻摇晃过夜，以便完全溶解DNA。 建议添加1 mM EDTA，以防止核酸酶降解DNA。
本品采用未指定性别的小牛的胸腺组织制备。
Analysis Note
“高度聚合”
Application
小牛胸腺DNA（CT-DNA）是一种天然DNA，广泛用于研究调节DNA结构和功能的DNA结合抗癌剂和DNA结合剂。 小牛胸腺DNA也用于溶液中DNA行为的物理化学研究。
General description
λmax 259 nm（100 mM磷酸盐缓冲液，pH7.0）。
来自小牛胸腺的DNA含41.9 mole % G-C和58.1 mole % A-T。1 在260nm处的OD为1.0，对应于约50 μg的双链DNA。
来自小牛胸腺的DNA含41.9 mole % G-C和58.1 mole % A-T。1 在260nm处的OD为1.0，对应于约50 μg的双链DNA。
Physical form
纤维状制剂
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
M S Hazelton et al.
Journal of dairy science, 103(12), 11844-11856 (2020-09-29)
Replacement dairy heifers exposed to Mycoplasma bovis as calves may be at risk of future clinical disease and pathogen transmission, both within and between herds; however, little information is available about these risks. We conducted a 2-yr longitudinal (panel) study
Alysia M Parker et al.
Veterinary microbiology, 196, 118-125 (2016-12-13)
Mycoplasma bovis is a major pathogen in cattle causing mastitis, arthritis and pneumonia. First isolated in Australian cattle in 1970, M. bovis has persisted causing serious disease in infected herds. To date, genetic analysis of Australian M. bovis isolates has
Fariba Mollarasouli et al.
Journal of pharmaceutical analysis, 10(5), 473-481 (2020-11-03)
In this study, an electrochemical DNA biosensor was developed using a straightforward methodology to investigate the interaction of indinavir with calf thymus double-stranded deoxyribonucleic acid (ct-dsDNA) for the first time. The decrease in the oxidation signals of deoxyguanosine (dGuo) and
M S Hazelton et al.
Journal of dairy science, 101(8), 7412-7424 (2018-05-14)
With the common use of bulls for breeding following a period of artificial insemination in seasonally bred dairy herds, it is important to consider the potential role of the bull in transmission of Mycoplasma spp. within and between herds. This
Hironobu Ikehata et al.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 17(4), 404-413 (2018-02-22)
The amount of photolesions produced in DNA after exposure to physiological doses of ultraviolet radiation (UVR) can be estimated with high sensitivity and at low cost through an immunological assay, ELISA, which, however, provides only a relative estimate that cannot
实验方案
Spectrophotometric assay at 260 nm measures nuclease S1 activity, vital for nucleic acid research, with defined enzyme unit criteria.
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