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生物来源
Escherichia coli
质量水平
表单
aqueous glycerol solution
分子量
~374 kDa
浓度
≥2 unit/μL
技术
cell based assay: suitable
应用
cell analysis
运输
dry ice
储存温度
−70°C
基因信息
Escherichia coli K12 ... gyrA(946614) , gyrB(948211)
应用
来自 大肠杆菌 的 DNA 旋转酶已用于研究比较蛋白质组学方法，以更好地定义拟核异常球菌的特异性。来自 大肠杆菌 的 DNA 旋转酶也用于研究DnaK-ClpB bichaperone系统在 DNA 旋转酶再激活中的作用。
生化/生理作用
DNA 旋转酶以A 2 B 2 全酶的形式提供。亚单位 A 的分子量为 97 kDa，亚单位 B 的分子量为 90 kDa。它催化负性超螺旋 ATP 依赖性引入松弛 DNA 这一过程。通过诱变实验，已成功地将 DNA 旋转酶转化为 II 类型拓扑异构酶。
可用于超螺旋质粒。
单位定义
在 37°C下，一个单位的旋转酶活性将在30分钟内超螺旋 0.5 微克的 pBR-322 DNA。
其他说明
溶于含 Tris 缓冲液、DTT 和 EDTA 的 50% 甘油中。
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
S C Kampranis et al.
Proceedings of the National Academy of Sciences of the United States of America, 93(25), 14416-14421 (1996-12-10)
DNA gyrase is unique among topoisomerases in its ability to introduce negative supercoils into closed-circular DNA. We have demonstrated that deletion of the C-terminal DNA-binding domain of the A subunit of gyrase gives rise to an enzyme that cannot supercoil
Alix Pantel et al.
Antimicrobial agents and chemotherapy, 56(4), 1990-1996 (2012-02-01)
Fluoroquinolone (FQ) resistance is emerging in Mycobacterium tuberculosis. The main mechanism of FQ resistance is amino acid substitution within the quinolone resistance-determining region (QRDR) of the GyrA subunit of DNA gyrase, the sole FQ target in M. tuberculosis. However, substitutions
Teresa Lara-Ortíz et al.
Canadian journal of microbiology, 58(2), 195-199 (2012-01-24)
In Escherichia coli cells, an increase in temperature induces immediate DNA relaxation, followed by the fast recovery of DNA supercoiling. DNA gyrase, proteins synthesized during heat stress, and chaperone DnaK have been proposed to participate in this recovery. However, the
Magali Toueille et al.
Journal of proteomics, 75(9), 2588-2600 (2012-03-27)
Compared to radiation-sensitive bacteria, the nucleoids of radiation-resistant Deinococcus species show a higher degree of compaction. Such a condensed nucleoid may contribute to the extreme radiation resistance of Deinococcus by limiting dispersion of radiation-induced DNA fragments. Architectural proteins may play
Adam B Shapiro
Biochemical pharmacology, 85(9), 1269-1277 (2013-02-19)
A novel, high-throughput-compatible assay for the ATP-dependent supercoiled DNA relaxing activity of human topoisomerase IIα (hTopoIIα) is described. The principle of detection is the preferential binding of the oligodeoxyribonucleotide BODIPY-TMR-5'-TTCTTCTTCT-3' to relaxed double-stranded plasmid containing the triplex forming sequence (TTC)9
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