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Merck
CN

D0672

Sigma-Aldrich

φX174 DNA Marker Hae III Digest

for DNA electrophoresis

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About This Item

UNSPSC代码:
41105335
NACRES:
NA.25

质量水平

100
200

形式

liquid

用途

200 uses (0.1 mg size)
40 uses (20 μg size)

适用性

suitable for electrophoresis (DNA)

储存温度

−20°C

一般描述

Sigma′s Phi X174 DNA Marker Hae III Digest contains 11 fragments from 72 − 1,353 bp. Five ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

组分

Sigma′s Phi X174 DNA Marker Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

制备说明

φX174RF-I DNA was digested with Hae III to completion, then purified by phenol extraction.

其他说明

For optimal resolution, the recommended agarose gel concentration is 2.0%.

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品

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商品

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

选择适合的标记物和分子量标准,用于测定电泳分离样品的核酸大小。通过琼脂糖或聚丙烯酰胺凝胶检测DNA、RNA和PCR生成片段的大小。

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