生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
ascites fluid
抗体产品类型
primary antibodies
克隆
DDC-109, monoclonal
分子量
antigen ~50 kDa
包含
15 mM sodium azide
种属反应性
rabbit, sheep, guinea pig, monkey, rat, human, bovine, canine
技术
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:1,000 using bovine brain extract
同位素/亚型
IgG2b
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... DDC(1644)
rat ... Ddc(24311)
一般描述
DDC is a 85-90 kDa molecular weight protein that is mainly expressed in brain, kidney and liver.
Monoclonal Anti-DOPA Decarboxylase (DDC) (mouse IgG2b isotype) is derived from the DDC-109 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
应用
Monoclonal Anti-DOPA Decarboxylase (DDC) antibody produced in mouse may also be used in immunoprecipitation, immunocytochemistry and ELISA applications. In immunocytochemical labeling of brain tissue, the product stains the monoaminergic cells and proximal dendrites, but not cortical fibers. It is suitable for use in western blotting at a working dilution of 1:1000 using bovine brain extract. It was used for immunoblotting and immunoprecipitation of AADC (aromatic amino acid decarboxylase) from rat striatum in a study. It was used as a primary antibody for western blotting and co-immunoprecipitation studies using human embryonic kidney cells and human neuroblastoma cells.
生化/生理作用
DOPA Decarboxylase (DDC), which requires pyridoxal phosphate for its activity, decarboxylates 5-hydroxytryptophan to form serotonin. It also decarboxylates L-DOPA to form dopamine in both dopaminergic and noradrenergic neurons.
DOPA decarboxylase (DDC, EC 4.1.1.28), catalyzes the decarboxylation of the 5-hydroxytrptophan (5-HTP) to 5-HT. It requires pyridoxal phosphate for its activity. This is the enzyme that also decarboxylates L-DOPA to form dopamine in both dopaminergic and noradrenergic.. Antibodies that react specifically with the enzyme enable the identification of both noradrenergic, dopaminergic, and serotoninergic systems, since DDC is present and active in all three types of neurons.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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