产品线
ColorWheel®
质量水平
形式
lyophilized
环保替代产品特性
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
技术
flow cytometry: suitable
荧光
λex 690 nm; λem 713 nm
相容性
for use with ColorWheel® Dye-ready Antibodies (Required, sold separately)
环保替代产品分类
, Aligned
运输
ambient
储存温度
2-8°C
一般描述
ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.
iFluor 700 succinimidyl ester is bright, photostable fluorescent dye with minimal quenching on proteins. Its spectral properties comparable to Alexa Fluor™ 700. It exhibits an excitation max at 690 nm and an emission max at 713 nm with an extinction coefficient of 220,000 cm-1 M-1. It displays good reactivity and selectivity with protein amine groups.
iFluor 700 succinimidyl ester is bright, photostable fluorescent dye with minimal quenching on proteins. Its spectral properties comparable to Alexa Fluor™ 700. It exhibits an excitation max at 690 nm and an emission max at 713 nm with an extinction coefficient of 220,000 cm-1 M-1. It displays good reactivity and selectivity with protein amine groups.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
应用
Quality Control Testing:
Flow Cytometry: Excitation: 690nm, Emission: 713nm
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Flow Cytometry: Excitation: 690nm, Emission: 713nm
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
相容性
Requires ColorWheel® Antibody (Sold Separately)
外形
CWDS-IF700-25UL Set Components
CWD-IF700-25UL - ColorWheel® Antibody-Ready iFluor 700 Dye
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
CWD-FINISH-25UL - ColorWheel® Finishing Buffer
Lyophilized from PBS. Contains no biocide or preservatives, such as azide.
CWD-IF700-25UL - ColorWheel® Antibody-Ready iFluor 700 Dye
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
CWD-FINISH-25UL - ColorWheel® Finishing Buffer
Lyophilized from PBS. Contains no biocide or preservatives, such as azide.
重悬
Reconstitute each vial by adding 25 μL PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.
法律信息
ALEXA FLUOR is a trademark of Life Technologies
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 3
法规信息
常规特殊物品
商品
查阅专家针对ColorWheel®流式抗体和染料常见问题(FAQ)所作的解答。
实验方案
ColorWheel®流式试剂3步法制备方案,让您在流式细胞术中体验到超强简便性。采用ColorWheel®流式抗体和染料,可轻松配制理想的流式细胞分析试剂,全程只需不到5分钟的实操时间。
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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