Anti-Mouse CD69 (H1.2F3) ColorWheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Clone H1.2F3 is an Armenian hamster monoclonal antibody that detects mouse CD69. It targets an epitope within the extracellular domain.

Mouse dendritic epidermal T cell line Y245

Quality Control Testing

Evaluated by Flow Cytometry in BALB/c mouse splenocytes.

Flow Cytometry Analysis: Staining of one million BALB/c mouse splenocytes was performed with 5 μL of a 1:1 mixture of Cat. No. CWA-1153, Anti-Mouse CD69 (H1.2F3) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1014 Armenian Hamster IgG isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Tested Applications

Flow Cytometry Analysis: Staining of one million BALB/c mouse splenocytes was performed with 5 μL of a 1:1 mixture of Cat. No. CWA-1153, Anti-Mouse CD69 (H1.2F3) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1014 Armenian Hamster IgG isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Early activation antigen CD69 (UniProt: P37217; also known as CD69) is encoded by the Cd69 gene (Gene ID: 12515) in murine species. CD69 is a single-pass type II membrane disulfide-linked homodimeric glycoprotein with a cytoplasmic domain (aa 1-40), a transmembrane domain (aa 41-61), and an extracellular domain (aa 62-199). It is a C-type lectin and a member of the natural killer (NK) receptor family. It is an early activation marker that is expressed in hematopoietic stem cells, activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells, and platelets. It is induced by antigens, mitogens, or activators of PKC on the surface of T and B-lymphocytes and by interaction of IL-2 with the p75 IL-2R on the surface of NK cells. CD69 is involved in immune cell homeostasis and regulates T cell-mediated immune response through the control of Th17 cell differentiation. Expression of CD69 by activated T lymphocytes is reported to trigger an anti-inflammatory mechanism mediated by Galectin-1, which regulates the immune response and prevents pathogenic Th17 responses. CD69 knockout mice are shown to develop an exacerbated form of collagen-induced arthritis. (Ref.: de la Fuente, H., et al. (2014). Mol. Cell. Biol. 34(13); 2479-2487; Ziegler SF et al., (1994). Stem Cells. 12(5); 456-65).

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.