Anti-Human LAMP-1/CD107a (2E9) ColorWheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

This ColorWheel^®Dye-Ready monoclonal antibody was developed using our proprietary ZooMAb^® recombinant antibody technology. It offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Clone 2E9 is a recombinant rabbit monoclonal antibody that detects LAMP1 (CD107a). It targets an epitope within 14 amino acids from the N-terminal, lumenal domain.

KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal, lumenal domain of human LAMP-1.

Quality Control Testing

Evaluated by Flow Cytometry in Fixed and permeabilized Jurkat cells.

Flow Cytometry Analysis: Intracellular staining of one million fixed and permeabilized Jurkat cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1146, Anti-Human LAMP-1/CD107a (2E9) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1053 Rabbit IgG isotype control and Cat. No. CWDS-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Tested Applications

Flow Cytometry Analysis: Intracellular staining of one million fixed and permeabilized Jurkat cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1146, Anti-Human LAMP-1/CD107a (2E9) ColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1053 Rabbit IgG isotype control and Cat. No. CWDS-APC ColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Lysosome-associated membrane glycoprotein 1 (UniProt: P11279; also known as LAMP-1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, CD107a) is encoded by the LAMP1 gene (Gene ID: 3916) in human. LAMP-1 and LAMP-2 are two highly glycosylated proteins found in the lysosomal membrane. They may contribute up to 50% of all proteins present in the lysosomal membranes. They are known to maintain the lysosomal acidification and lysosomal membrane integrity. Extensive N-glycosylation on LAMP-1 and 2 to protects them from the lysosomal proteolysis. However, higher levels of poly-N-acetyllactosamines on LAMP-1 and 2 have been correlated with decreased cellular differentiation and enhanced metastatic potential. Over-expression of LAMP-1 has also been implicated in breast cancer development and progression. LAMP-1 is a single-pass type I transmembrane protein that is synthesized with a signal peptide (aa 1-28), which is subsequently cleaved off to generate the mature form that contains a large lumenal domain (aa 29-382), a transmembrane domain (aa 383-405), and a short C-terminal cytoplasmic tail (aa 406-417). LAMP-1 presents carbohydrate ligands to selectins and is also reported to act as a receptor for Lassa virus protein. It is reported to shuttle between lysosomes, endosomes, and the plasma membrane. Two isoforms of LAMP-1 have been described that are produced by alternative splicing. (Ref.: Wang, Q., et al. (2017). Oncol. Lett. 14(4); 4729 - 4735; Tan, K-P., et al. (2016). Cell Death Dis. 7, e2347).

This ColorWheel^® Dye-Ready monoclonal antibody was developed using our ZooMAb^® recombinant monoclonal antibody (Cat. No. ZRB1131). It offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.