Anti-Mouse CD24 (M1/69) ColorWheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Clone M1/69 is a rat monoclonal antibody that detects murine CD24.

Spleen cells from B 10 mice enriched for T lymphocytes and spleen cells treated with 1 pg/ml concanavalin.

Quality Control Testing:

Evaluated by Flow Cytometry in C57Bl/6 mouse splenocytes.

Staining of one million mouse splenocytes was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1054, Anti-Mouse CD24 (M1/69) ColorWheel^® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Rat IgG2b isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Signal transducer CD24 (UniProt: P24807; also known as Lymphocyte antigen 52, Ly-52, M1/69-J11D heat stable antigen,HSA, Nectadrin, R13-Ag, X62 heat stable antigen, CD24) is encoded by the Cd24 (also known as Cd24a, Ly-52) gene (Gene ID: 12484) in murine species. CD24 is a GPI-anchor protein without any intracellular signaling domain. It plays a pivotal role in differentiation of different cell types. Its expression is reported on hematopoietic cells, including B cells, T cells, neutrophils, dendritic cells, eosinophils, and macrophages. It is also expressed in several non-hematopoetic cells, including neuronal cells, keratinocytes, pancreatic cells, and several types of cancer cells. Its expression is shown to be higher in progenitor cells and metabolically active cells and to a lesser extent in terminally differentiated cells. In association with SIGLEC10, CD24 is reported to be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs), such as the high mobility group box protein 1 )HMGB1), HSP70, and HSP90. CD24 also plays a role in T cell survival. In the absence of CD24, homeostatic proliferation of T cells is markedly reduced, however immune-driven proliferation is less affected. (Ref.: Fang, X., et al. (2010). Cell. Mol. Immunol. 7(2); 100-103).

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.