Anti-Human CD16 (3G8) ColorWheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Clone 3G8 is a mouse monoclonal antibody that detects human CD16.

Human neutrophils

Quality Control Testing:

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis: Staining of one million Human peripheral blood mononuclear cells (PBMC) was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1031, Anti-Human CD16 (3G8) ColorWheel^® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Mouse IgG1 isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Low affinity immunoglobulin gamma Fc region receptor III-A (UniProt: P08637; also known as CD16a antigen, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2, CD16a) is encoded by the FCGR3A (also known as CD16A, FCG3, FCGR3, IGFR3) gene (Gene ID: 2214) in human. CD16 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa1-16), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 17-208), a transmembrane domain (aa 209-229), and a cytoplasmic domain (aa 230-254). It contains high mannose- and complex-type oligosaccharides and its Glycosylation at Asn-180 is shown to be essential for high affinity binding to the Fc and for discrimination between fucosylated and afucosylated IgG glycoforms. It is expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes, and placental trophoblasts. CD16 serves as a receptor for the Fc region of IgG and binds complexed or aggregated IgG and also monomeric IgG. Phenylalanine 203 in CD16a determines the transmembrane domains and serine 203 in CD16b determines the GPI-anchoring. CD16 is reported to mediate antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis and cytokine production. Mutations in FCGR3A gene have been linked to immunodeficiency 20 that is characterized by functional deficiency of NK cells and makes subjects vulnerable to severe herpes viral infections.

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.