Anti-Human CD64 (10.1) Colorwheel^® Dye-Ready mAb

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

High affinity immunoglobulin gamma Fc receptor I (UniProt: P12314; also known as IgG Fc receptor I, Fc-gamma RI, FcRI, Fc-gamma RIA, FcgammaRIa, CD64) is encoded by the FCGR1A (also known as FCG1, FCGR1, IGFR1) gene (Gene ID: 2209) in human. CD64 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-15), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 16-292), a transmembrane domain (aa 293-313), and a cytoplasmic domain (aa 314-374). Within its extracellular domain are three Ig-like C2 type domains. Its expression has been reported in monocytes/macrophages. Expression of CD64 distinguishes conventional dendritic cells from monocyte-derived dendritic cells. It serves as a high-affinity receptor for the Fc domains of IgG (FcR) and plays a key role in linking the humoral and the cellular arms for the immune system. It binds human IgG1, IgG3, and IgG4 with a high affinity, but does not show any affinity for IgG2. It functions in both innate and adaptive immune responses. Along with the low affinity receptor (FcRII), it mediates a variety of biological responses, including endocytosis, phagocytosis, antibody-directed cellular cytotoxicity, and cytokine release. Clone 10.1 is shown to react with an epitope on FcRI near to the binding site for the Fc region of IgG. (Ref.: Harrison, PT., and Allen., (1998). Protein Engineering. 11 (3); 225-232; Dougherty, GJ., et al. (1987). Eur. J. Immunol. 17(10); 1453-1459).

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Human rheumatoid synovial fluid cells and fibronectin purified monocytes.

Quality Control Testing

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis (FC): Staining of one million Human peripheral blood mononuclear cells (PBMC) was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1026, Anti-Human CD54 (10.1) ColorWheel^® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount PE-conjugated Mouse IgG1 isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Clone 10.1 is a mouse monoclonal antibody that detects CD64 (FCgR1). It targets an epitope within the extracellular domain.

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.