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用途
sufficient for 200 assays (96-well plates)
sufficient for 40 assays (1 mL cuvettes)
质量水平
运输
dry ice
储存温度
−20°C
基因信息
human ... CPA3(1359)
一般描述
Carboxypeptidase A (CPA) catalyzes the hydrolysis of C-terminal aromatic or aliphatic amino acids of proteins or peptides. Carboxypeptidase A is found in the pancreas and mast cells. Carboxypeptidase A in mast cells (MC-CPA) is a highly conserved secretory granule protease that is important for mast cell differentiation. MC-CPA levels rise during anaphylaxis, a severe acute allergic reaction. This enzyme is considered to be a sensitive marker for anaphylaxis, much more sensitive than the present marker tryptase. It has been suggested that MC-CPA can degrade snake venom components and honeybee venoms, thus having a protective function during envenomation.
应用
The Carboxypeptidase A Assay Kit allows for the determination of CPA activity in biological samples, as well as screening for enzyme inhibitors. The kit contains all the necessary components including a carboxypeptidase A control enzyme and carboxypeptidase inhibitor.
单位定义
1 unit will hydrolyze 1 μmole of N-(4-methoxyphenylazoformyl)-Phe-OH per minute at pH 8 at 25 °C.
分析说明
The CPA activity assay is based on the hydrolysis of the substrate N-(4-methoxyphenylazoformyl)-Phe-OH. The substrate has a strong absorption peak at 350 nm (εmM = 19.0). The reaction progression is reflected by a decrease in absorption at 350 nm.
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2
储存分类代码
10 - Combustible liquids
法规信息
新产品
W L Mock et al.
Analytical biochemistry, 239(2), 218-222 (1996-08-01)
N-(4-Methoxyphenylazoformyl)-L-phenylalanine is efficiently cleaved by the enzyme bovine carboxypeptidase A into fragments anisole, molecular nitrogen, carbonate, and phenylalanine, in the course of which an intense spectral absorption of the substrate (epsilon350 = 19,000 M-1 cm-1) disappears completely. This furnishes a
Mast cell carboxypeptidase as a new clinical marker for anaphylaxis.
Zhou, X., et al.
The Journal of Allergy and Clinical Immunology, 117, S85-S85 (2006)
Martin Metz et al.
Science (New York, N.Y.), 313(5786), 526-530 (2006-07-29)
Snake or honeybee envenomation can cause substantial morbidity and mortality, and it has been proposed that the activation of mast cells by snake or insect venoms can contribute to these effects. We show, in contrast, that mast cells can significantly
Thorsten B Feyerabend et al.
Molecular and cellular biology, 25(14), 6199-6210 (2005-07-01)
Mast cell carboxypeptidase A (Mc-cpa) is a highly conserved secretory granule protease. The onset of expression in mast cell progenitors and lineage specificity suggest an important role for Mc-cpa in mast cells. To address the function of Mc-cpa, we generated
Tryptase-PAR2 axis in experimental autoimmune prostatitis, a model for chronic pelvic pain syndrome.
Kenny Roman et al.
Pain, 155(7), 1328-1338 (2014-04-15)
Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) affects up to 15% of the male population and is characterized by pelvic pain. Mast cells are implicated in the murine experimental autoimmune prostatitis (EAP) model as key to chronic pelvic pain development. The
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