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生化/生理作用
Cathepsin D, a ubiquitous aspartic protease belonging to the A1 peptidase family, is found intracellularly in lysosomes. The enzyme has been associated with various biological processes such as: apoptotic events (e.g., the release of cytochrome c from mitochondria and the loss of the transmembrane potential (ΔΨ), aging, Alzheimer′s disease, and breast cancer.
特点和优势
- Contains all the reagents required for the measurement of the cathepsin D activity in cell and tissue lysates.
- The bottom limit of detection is 750 pg of protein (or 13 femtomoles of enzyme).
- A single step reaction with direct, real time observation of the degradation product released by the enzyme.
- The kit has been tested on extracts from CHO, HeLa, HEK 293, A431, HepG2, U937, and Jurkat cells on S. cerevisiae and on rat brain, kidney and liver tissues.
分析说明
The assay is based on the hydrolysis, by the enzyme, of an internally quenched fluorimetric substrate.
仅试剂盒组分
产品编号
说明
- Assay Buffer 1x 20 mL
- Cathepsin D from bovine spleen 5 U
- Albumin Solution 1 mL
- Cathepsin D Substrate Solution .25 mL
- 7-Methoxycoumarin 4-acetic acid Standard Solution .1 mL
- Pepstatin A Inhibitor Solution .6 mL
警示用语：
Danger
危险声明
危险分类
Aquatic Acute 1 - Aquatic Chronic 1 - Skin Corr. 1B
储存分类代码
8B - Non-combustible corrosive hazardous materials
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