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Merck
CN
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文件

安全信息

CS0790

Sigma-Aldrich

抗氧化检测试剂盒

sufficient for 200 tests

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别名:
Oxidative Stress Detection Kit
UNSPSC代码:
12161503
NACRES:
NA.84

用途

sufficient for 200 tests

质量水平

200

检测方法

colorimetric

运输

wet ice

储存温度

2-8°C

相关类别

一般描述

在存在抗氧化剂的条件下，自由基阳离子受抑制的程度取决于抗氧化剂活性，且颜色深度成比例下降。Trolox是一种水溶性维生素E类似物，可作为标准抗氧化剂或对照抗氧化剂。
自由基或活性氧(ROS)在生化氧化还原反应过程产生，是正常生理细胞新陈代谢（免受传染性生物体侵害）的参与成分，并可对紫外线、香烟烟雾、环境污染物和伽马辐射等环境因素作出响应。ROS形成之后即会攻击细胞成分，破坏脂质、蛋白质和DNA，从而引发多种疾病，包括癌症、动脉粥样硬化、类风湿性关节炎、糖尿病、肝损伤和中枢神经系统疾病。

应用

它已在A431和CHO细胞裂解物，大鼠脑、肝和肾裂解物，人血浆、血清、尿液和唾液中进行了测试。
抗氧化测定试剂盒提供了有效测定血浆、血清、尿液、唾液、细胞和组织裂解液的总抗氧化能力所需的所有试剂。它也被用来测定自由基阳离子（2,2′-连氮基-二-[3-乙基苯并噻唑啉磺酸盐]）（ABTS +）的清除能力。

适用性

适于测定血浆、血清、尿液、唾液、细胞和组织裂解液的总抗氧化能力

原理

抗氧化剂测定的原理是：由肌红蛋白和过氧化氢形成亚铁肌红蛋白自由基，该自由基将ABTS（2,2¢-联氮双(3-乙基苯并噻唑啉-6-磺酸）氧化生成自由基阳离子ABTS·+（绿色可溶性色原），从而在405 nm处用分光光度法测定。

制备说明

试剂用超纯水配置。

重悬

肌红蛋白溶液保存在-20℃条件下。
Trolox工作溶液保存在-20℃条件下。
ABTS底物溶液可在4℃下保存两周或-20℃下保存1个月。

法律信息

仅试剂盒组分

产品编号
说明
  • Assay Buffer 10X 30 mL
  • Stop Solution 20 mL
  • Hydrogen Peroxide, 3% solution 1 mL
  • Myoglobin from horse heart 2 x 1
  • (±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox®) 2 x 1
  • ABTS, 10 mg tablets 4 tab
  • Phosphate-Citrate Buffer, pH 5 4 tab

相关产品

产品编号
说明
价格

象形图

CorrosionExclamation mark
GHS05,GHS07

警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物源性产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the pH of kit component A3605 in the Antioxidant Assay Kit, product CS0790?

    The pH of kit component A3605 in the Antioxidant Assay Kit, product CS0790, is approximately 5,  at 25°C. The adequacy/suitability of the pH of the assay buffer is assessed via the assay itself, and is tested for each lot of kits.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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