用途
sufficient for 50 reactions (1 mL)
sufficient for 500 reactions (100 μL)
质量水平
运输
wet ice
储存温度
2-8°C
基因信息
human ... NAGLU(4669)
应用
该试剂盒可在细胞溶解液、组织匀浆或纯化酶等样品中方便、快速地检测β-N-乙酰氨基葡萄糖苷酶。它是一种理想的分离细胞/组织样品溶酶体检测工具。
生化/生理作用
哺乳类动物体内的N-乙酰-D-氨基葡萄糖苷酶 (NAG) 是一种参与胞内糖脂和糖蛋白降解的溶酶体酶。在人类肾脏、肺和肝脏溶酶体中都已检测到高活性的NAG。血清NAG水平升高与某些癌症有关。
分析说明
该试剂盒的检测原理为NAG（NP-GLcNAc）底物在酶促作用下水解。酶水解底物后，释放出对硝基苯胺离子。然后在405 nm处采用比色法测定反应产物。
仅试剂盒组分
产品编号
说明
- Dilution Buffer 8 mL
- 4-Nitrophenyl-N-acetyl-β-D-glucosaminide 50 mg
- Citrate Buffer Solution, 0.09 M 100 mL
- p-Nitrophenol Standard Solution, 10 mM 1 mL
- β-N-Acetylglucosaminidase from Jack beans 1 vial
- Sodium carbonate 5 g
相关产品
警示用语：
Warning
危险声明
危险分类
Carc. 2 - Eye Irrit. 2 - STOT RE 2 Oral
靶器官
Liver,Kidney
储存分类代码
11 - Combustible Solids
法规信息
易制毒化学品（2类）
含少量动物源组分生物产品
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商品
亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。
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