usage
sufficient for 20 purifications
shipped in
dry ice
storage temp.
−20°C
Application
该试剂盒提供了一种从组织培养细胞中获取富含小窝/脂筏蛋白的级分的简单方法。这种分离小窝和脂筏的方法是基于这些结构在冰冷的非离子洗涤剂 Triton X-100 中的不溶性。该试剂盒包括用于细胞裂解、密度梯度和分离小窝/脂筏蛋白富集部分的所有试剂，以及小窝/脂筏区室的两个标记物，抗-小窝蛋白-1和霍乱毒素 B 亚基过氧化物酶（CTB-HRP ）。
Biochem/physiol Actions
小窝和脂筏是重要的富含胆固醇和鞘脂的结构，它们代表质膜的亚区室或微区，并与细胞转运和信号转导相关事件有关。
仅试剂盒组分
产品编号
说明
- Lysis buffer 100 mL
- Cholera Toxin B Subunit, Peroxidase (CTB-HRP) 50 μg
- Protease inhibitor cocktail for use with mammalian cell and tissue extracts 1 mL
- Anti-Caveolin-1 antibody produced in rabbit 50 μL
- Triton X-100 2 mL
- OptiPrep Density Gradient Medium 100 mL
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
存储类别
10 - Combustible liquids
法规信息
高风险级别生物产品--毒素类产品
此项目有
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