推荐产品
用途
sufficient for 20 purifications
运输
dry ice
储存温度
−20°C
应用
该试剂盒提供了一种从组织培养细胞中获取富含小窝/脂筏蛋白的级分的简单方法。这种分离小窝和脂筏的方法是基于这些结构在冰冷的非离子洗涤剂 Triton X-100 中的不溶性。该试剂盒包括用于细胞裂解、密度梯度和分离小窝/脂筏蛋白富集部分的所有试剂，以及小窝/脂筏区室的两个标记物，抗-小窝蛋白-1和霍乱毒素 B 亚基过氧化物酶（CTB-HRP ）。
生化/生理作用
小窝和脂筏是重要的富含胆固醇和鞘脂的结构，它们代表质膜的亚区室或微区，并与细胞转运和信号转导相关事件有关。
仅试剂盒组分
产品编号
说明
- Lysis buffer 100 mL
- Cholera Toxin B Subunit, Peroxidase (CTB-HRP) 50 μg
- Protease inhibitor cocktail for use with mammalian cell and tissue extracts 1 mL
- Anti-Caveolin-1 antibody produced in rabbit 50 μL
- Triton X-100 2 mL
- OptiPrep Density Gradient Medium 100 mL
警示用语：
Danger
危险分类
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
WGK
WGK 3
法规信息
高风险级别生物产品--毒素类产品
Chiung-Chun Huang et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 34(8), 1979-1992 (2009-02-13)
Neural adaptations in the medial prefrontal cortex (mPFC) are thought to be crucial in the development and maintenance of addictive behaviors. The mPFC receives a dense serotonergic (5-hydroxytryptamine, 5-HT) innervation from raphe nuclei and 5-HT exerts complex actions on mPFC
Ambrose Jong et al.
Cellular microbiology, 10(6), 1313-1326 (2008-02-06)
Pathogenic yeast Cryptococcus neoformans causes devastating cryptococcal meningoencephalitis. Our previous studies demonstrated that C. neoformans hyaluronic acid was required for invasion into human brain microvascular endothelial cells (HBMEC), which constitute the blood-brain barrier. In this report, we demonstrate that C.
Sheng-He Huang et al.
The Journal of biological chemistry, 286(40), 34761-34769 (2011-06-23)
Cryptococcus neoformans is a neurotropic fungal pathogen, which provokes the onset of devastating meningoencephalitis. We used human brain microvascular endothelial cells (HBMEC) as the in vitro model to investigate how C. neoformans traverses across the blood-brain barrier. In this study
Xing Wang et al.
PLoS pathogens, 13(9), e1006580-e1006580 (2017-09-29)
There is increasing consensus that males are more vulnerable than females to infection by several pathogens. However, the underlying mechanism needs further investigation. Here, it was showed that knockdown of androgen receptor (AR) expression or pre-treatment with 5α-dihydrotestosterone, the AR
Richard G Carroll et al.
The Journal of biological chemistry, 293(15), 5509-5521 (2018-02-22)
Different immune activation states require distinct metabolic features and activities in immune cells. For instance, inhibition of fatty acid synthase (FASN), which catalyzes the synthesis of long-chain fatty acids, prevents the proinflammatory response in macrophages; however, the precise role of
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门