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用途
sufficient for 50 reactions
运输
dry ice
储存温度
−20°C
一般描述
该试剂盒的检测原理为使用特异的酪氨酸激酶抗体，免疫沉淀目标酪氨酸激酶。在存在(γ32P)-ATP的条件下，将免疫沉淀的激酶与其底物(poly [Glu, Tyr] 4:1)一同孵育。然后测定掺入的放射性磷酸含量。
生化/生理作用
蛋白质酪氨酸激酶(PTK)参与调节细胞间有关生长、分化、贴壁、迁移和死亡的信号传导。在糖尿病、癌症等诸多疾病的发展过程中，都发挥非常重要的作用。
分析说明
本试剂盒包含过氧化物酶偶联的抗磷酸酪氨酸抗体，可通过斑点印迹法来分析底物。该检测可作为一种快捷的替代性非放射性检测方法。
仅试剂盒组分
产品编号
说明
- Monoclonal Anti-Phosphotyrosine-Peroxidase antibody produced in mouse 1 vial
- Wash Buffer 10X 8 mL
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- P81 Cellulose Phosphate Squares 10 ea
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