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质量水平
用途
kit sufficient for 100 assays (1 mL)
运输
dry ice
储存温度
−20°C
基因信息
human ... TXNRD1(7296) , TXNRD2(10587) , TXNRD3(114112)
一般描述
硫氧还蛋白还原酶检测试剂盒采用比色法对哺乳动物细胞核组织中的硫氧还蛋白还原酶进行检测。该试剂盒原理是硫氧还蛋白还原酶可催化NADPH使5,5′-二硫双(2-硝基苯甲酸) (DTNB)还原成5-硫代-2-硝基苯甲酸(TNB)，后者在412nm处呈现强黄色。
DTNB + NADPH + H+ ↔ 2 TNB + NADP+
DTNB + NADPH + H+ ↔ 2 TNB + NADP+
应用
使用大鼠肝去除细胞核后的提取物，本试剂盒的线性度可达0.25单位/ml。在该试剂盒反应中，约20%的活性是非特异性还原酶的活性。(TrxR抑制剂对其无效).
特点和优势
- 检测方法简单易行。
- 试剂盒中包含了比色法检测哺乳动物硫氧还蛋白还原酶所需的所有试剂。
- 试剂盒中提供了特异性抑制哺乳动物硫氧还蛋白还原酶的溶液。
- 该试剂盒已通过心肌和HeLa, A549, Jurkat, U937, A431, COS, CHO 和 NIH 3T3 等细胞系裂解物的测试。
仅试剂盒组分
产品编号
说明
- Assay Buffer 5x for Thioredoxin Reductase 30 mL
- Thioredoxin Reductase 10 μg
- Thioredoxin Reductase Inhibitor Solution .05 mL
- 5,5'-Dithiobis(2-nitrobenzoic) acid (DNTB) 150 mg
- NADPH 25 mg
- Dimethyl Sulfoxide (DMSO) 7.5 mL
警示用语：
Warning
危险声明
危险分类
Repr. 2
储存分类代码
10 - Combustible liquids
闪点(°F)
188.6 °F
闪点(°C)
87 °C
法规信息
动植物源性产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Matthew T Harper
Platelets, 30(1), 98-104 (2017-12-02)
Platelets are central to normal hemostasis and must be tightly controlled to prevent thrombosis. However, drug treatments that also affect platelets could lead to unwanted side effects on hemostasis or thrombosis. In this study, the effect of auranofin on platelets
Tessa D Epstein et al.
ACS infectious diseases, 5(10), 1682-1687 (2019-09-06)
Helicobacter pylori (H. pylori) infection poses a worldwide public health crisis, as chronic infection is rampant and can lead to gastric ulcers, gastritis, and gastric cancer. Unfortunately, frontline therapies cause harmful side effects and are often ineffective due to antibiotic resistance.
Francesca Magherini et al.
Oncotarget, 9(46), 28042-28068 (2018-07-03)
Au(NHC) and Au(NHC)2, i.e. a monocarbene gold(I) complex and the corresponding bis(carbene) complex, are two structurally related compounds, endowed with cytotoxic properties against several cancer cell lines. Herein, we explore the molecular and cellular mechanisms at the basis of their
Ida Landini et al.
Journal of inorganic biochemistry, 208, 111079-111079 (2020-06-05)
The antiproliferative properties of a series of structurally-related gold(I) and silver(I) linear complexes inspired to the clinically established gold-based drug auranofin were investigated in A2780 ovarian cancer cells and in their auranofin (A2780/AF-R) and cisplatin (A2780/CDDP-R) resistant counterparts. In A2780
Iwona Jarocka-Karpowicz et al.
Biomolecules, 10(3) (2020-03-04)
Psoriasis is a chronic inflammatory skin disease characterized by dysregulated keratinocyte differentiation, but oxidative stress also plays an important role in the pathogenesis of this disease. Here, we examined the effect of cannabidiol (CBD), a phytocannabinoid with antioxidant and anti-inflammatory
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