产品名称
CRISPR Activator Human Oct4 Positive Control Plasmid, gRNA Only Expression Plasmid
form
liquid
packaging
vial of 50 μL
concentration
20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
application(s)
CRISPR
shipped in
dry ice
storage temp.
−20°C
Quality Level
Application
Functional Genomics/Target Validation
- Epigenetic Modification
- Transcriptional Activation
Features and Benefits
Serves as an experimental control for the CRISPR dCas9-p300 activation system. A positive experiment will result in an increased level of mRNA via qPCR or TaqMan PCR assay.
General description
OCT4 is the key transcription factor (TF) that maintains pluripotency of embryonic stem cells and plays a critical role in reinstating cellular pluripotency. The expression of OCT4 is stringently silenced in differentiated cells. Activation of silenced OCT4 gene has become a hallmark event during epigenetic reprogramming into iPSCs. The positive control OCT4 gRNA design has been tested with the dCas9-p300 plasmid in HEK293 cells and shown to increase OCT4 mRNA transcript levels over three-fold compared to a negative control.
Legal Information
Other Notes
1 vial containing 1ug of U6-gRNA plasmid expressing a guide sequence to human OCT4 (Octamer-binding transcription factor-4 or POU5F1).
Must be used with dCas9-p300 or other dCas9 activator/repressor plasmids.
To order U6-gRNA plasmids, please visit sigma.com/crisprs
Click here for more information on dcas9-p300 Gene Activator Expression Plasmid.
Click here for more information on dcas9-p300 Gene Activator Expression Plasmid.
存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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