形式
liquid
质量水平
100
200
包装
vial of 50 μL
浓度
20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
应用
CRISPR
运输
dry ice
储存温度
−20°C
一般描述
The Sigma lenti CRISPR Non-Targeting Control is a lentiviral plasmid vector, which includes a gRNA sequence that does not target known human, mouse and rat genes. This vector is useful as a negative control in experiments using Sigma CRISPR lentiviral clones. The Sigma lenti CRISPR non-targeting control uses a one-plasmid system consisting of a EF1-alpha-driven Cas9, a U6-driven guide RNA (non-targeting), with both Puromycin and GFP co-expressed with Cas9.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. Also, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (Product No. SHP001).
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas), originally found in bacteria and archaea, is employed by organisms to protect them from invading viruses and plasmids.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. Also, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (Product No. SHP001).
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas), originally found in bacteria and archaea, is employed by organisms to protect them from invading viruses and plasmids.
应用
Functional Genomics/Screening/Target Validation
To see more application data, protocols, vector maps visit sigma.com/crisprs.
- Non-targeting control, CRISPR lentivirus DNA format, for creating gene knockouts/genetic modifications in cell lines.
To see more application data, protocols, vector maps visit sigma.com/crisprs.
特点和优势
Serves as a non-targeting or negative experimental control (DNA format) for the CRISPR editing workflow using lenti CRISPRs. This allows you to examine the effect of transfection in your system with the Sigma lenti CRISPR/Cas9 plasmid.
外形
The Sigma CRISPR Lenti Non-Targeting Control Vector is provided as 1 μg of plasmid DNA in Tris-EDTA (TE).
法律信息
CRISPR专利正在申请中
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Basit Salik et al.
Cancer cell, 38(2), 263-278 (2020-06-20)
Signals driving aberrant self-renewal in the heterogeneous leukemia stem cell (LSC) pool determine aggressiveness of acute myeloid leukemia (AML). We report that a positive modulator of canonical WNT signaling pathway, RSPO-LGR4, upregulates key self-renewal genes and is essential for LSC
实验方案
Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.
了解CRISPR Cas9、定义和工作原理。CRISPR是一种全新、实惠的基因组编辑工具，可让所有人掌握基因组编辑。
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