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安全信息

CN402-05

Sigma-Aldrich

Canine Chondrocytes: CnC (Cryovial)

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About This Item

UNSPSC代码:
41106514
NACRES:
NA.81

生物来源

canine articular cartilage (normal)

包装

pkg of 500,000 cells

制造商/商品名称

Cell Applications, Inc

生长模式

Adherent

核型

2n = 78

形态学

chondrocyte

技术

cell culture | mammalian: suitable

相关疾病

arthritis

运输

dry ice

储存温度

−196°C

一般描述

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Canine Chondrocytes (CnC) are derived from normal canine articular cartilage where they produce and maintain the extracellular matrix of cartilage. Following monolayer culturing, CnC can be grown in alginate microspheres to study chondrocyte proliferation and metabolism in a three-dimensional in vitro system. Alginate microspheres provide more physiological conditions for the chondrocytes and allow them to sustain normal proteoglycans production and retention. Because of the prevalence of degenerative joint disease in geriatric dogs, CnC can be a useful tool for veterinary practice.

Canine Chondrocytes (CnC) were utilized to evaluate anti-inflammatory and anti-oxidant activity of Glyco-Flex III (Yáñez, 2008) and trans-pterostilbene (Remsberg, 2008). They were also used in material studies aimed to develop optimal surfaces/scaffolds for engineered tissues, cellular arrays, biosensors and lab-on-chip devices (Wright, 2012; Shaik 2013a,b).

细胞系来源

Cartilage

应用

production and maintenance of extracellular matrix, cartilage, collagen, differentiation and de-differentiation, signal transduction, apoptosis, differentiation, drug screening, gene expression, cytokine production, agarose assays, chondrocyte adhesion to medical implants, scaffolds for cartilage regeneration

组分

Canine Chondryocyte Basal Medium containing 10% FBS & 10% DMSO

制备说明

  • 1st passage, >500,000 cells in Canine Chondryocyte Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 10 doublings

传代培养常规

Please refer to the CnC Culture Protocol.

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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