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安全信息

CMC0024

Sigma-Aldrich

CONTROLLER BL21(DE3)化学感受态细胞

for controlled protein expression

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UNSPSC代码:
12352200
NACRES:
NA.85

生物来源

Escherichia coli

等级

for molecular biology

生长模式

adherent or suspension

形态学

rod shaped

技术

microbiological culture: suitable

细胞转化

competent cell type: chemically competent
transformation efficiency: >1 x 107 cfu/μg

运输

dry ice

储存温度

−70°C

一般描述

The CONTROLLER BL21(DE3) Chemically Competent Cells are an E. coli strain optimized for high efficiency transformation.
They are ideal for cloning and propagation of plasmid clones. They give high yield and high quality plasmid DNA.
Genotype

F- ompT hsdSB (rB- mB-) gal dcm (DE3) Mini-F lacIq1(GentR)

特点和优势

  • Reduce leaky expression: The LacI repressor protein binds to the lacO operator to block transcription of downstream genes. In the absence of an induction agent such as lactose sugar or IPTG, target protein expression from the adjacent promoter will be greatly reduced.
  • High protein yields: Both 10G and BL21(DE3)-based strains are noted for their ability to express high levels of protein. For expression from a T7 promoter please select the HI-Control BL21(DE3) strain. This strain also is deficient in proteases, yielding more stable protein preparations. Most other promoters can be used in either HI-Control strain.
  • Terrific efficiency: The simple, reliable heat-shock procedure for chemical transformation gives excellent results from small amounts of plasmid DNA.

组分

  • CONTROLLER BL21(DE3) chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression


WGK

WGK 3

法规信息

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M S Nadeem et al.
Brazilian journal of biology = Revista brasleira de biologia, 82, e244735-e244735 (2021-06-03)
L-Asparaginase catalysing the breakdown of L-Asparagine to L-Aspartate and ammonia is an enzyme of therapeutic importance in the treatment of cancer, especially the lymphomas and leukaemia. The present study describes the recombinant production, properties and anticancer potential of enzyme from

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