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CMC0006

Sigma-Aldrich

SIG10 ULTRA Electrocompetent Cells

for BAC & cosmid cloning

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About This Item

UNSPSC代码:
12352200
NACRES:
NA.85

生物来源

Escherichia coli

等级

for molecular biology

形式

buffered aqueous solution

生长模式

adherent or suspension

形态学

rod shaped

技术

microbiological culture: suitable

细胞转化

competent cell type: electrocompetent
transformation efficiency: ≥4 x 1010 cfu/μg

运输

dry ice

储存温度

−70°C

一般描述

The SIG10 ULTRA Electrocompetent Cells have the highest transformation efficiency available. They are to be used for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants possible.

Genotype

F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697galU galK rpsL nupG λ- tonA (StrR)

特点和优势

  • share the most useful genetic elements of standard cloning strains like DH5α™ DH10B, JM109, TOP10, etc. and directly replace them in cloning protocols.
  • provide the performance researchers need with ease of use.
  • provide solutions for a wide range of applications at economical prices.
  • Blue - white screening

组分

  • SIG10 ULTRA electrocompetent cells
  • pUC 19 transformation control DNA
  • recovery medium for cloning

法律信息

DH5α is a trademark of Invitrogen Corp.

WGK

WGK 3

法规信息

高风险级别生物产品--病毒，疫苗等

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