MCP21

Mouse Sp2/0 x Mouse BALB/c spleen

Antibodies secreted by the hybridoma line MCP21 react with subunit C3 in human and rabbit proteasomes, both with intact proteasomes and on blots. The hybridoma has been produced by repeated intraperitoneal immunisation of BALB/c mice with 20-50 μg of human placenta proteasomes and subsequent fusion of spleen cells with myeloma Sp2/0.

Human placenta proteasomes

Detection and purification of proteasomes and analysis of subunits

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 5x10,000 and 1x1,000,000 cells/ml; 5% CO₂; 37°C

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