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生物来源
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
骨髓瘤
Sp2/0
反应性
Anti-Proteasomes, human
技术
cell culture | mammalian: suitable
同位素/亚型
IgG1
运输
dry ice
储存温度
−196°C
相关类别
细胞系来源
Mouse Sp2/0 x Mouse BALB/c spleen
细胞系描述
Antibodies secreted by the hybridoma line MCP21 react with subunit C3 in human and rabbit proteasomes, both with intact proteasomes and on blots. The hybridoma has been produced by repeated intraperitoneal immunisation of BALB/c mice with 20-50 μg of human placenta proteasomes and subsequent fusion of spleen cells with myeloma Sp2/0.
免疫原
Human placenta proteasomes
应用
Detection and purification of proteasomes and analysis of subunits
培养基
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
传代培养常规
Maintain cultures between 5x10,000 and 1x1,000,000 cells/ml; 5% CO2; 37°C
其他说明
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