产品名称
TM4, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88111401, mouse testis, Not specified
biological source
mouse testis
packaging
tube of 5 μg 88111401-DNA-5UG
pkg of vial of cells 88111401-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Transferrin, H-Y Ag, novel retinol-binding protein
receptors
Androgen, oestrogen and progesterone receptors
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Hormone studies
Biochem/physiol Actions
From primary culture of sertoli cell enriched preparations from normal testes of a 11-13 day old BALB/c nu/+ mice. Cells respond to FSH but not LH; Plasminogen activator secretion is stimulated by FSH and also retinoic acid. Note: only distributed frozen.
Mouse BALB/c testis sertoli cell
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Preparation Note
Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:20 to 1:200 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cells resuscitated from frozen stock should be split 1:3 at the first passage and thereafter as above.
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