推荐产品
生物来源
mouse testis
生长模式
Adherent
核型
Not specified
形态学
Not specified
产品
Transferrin, H-Y Ag, novel retinol-binding protein
受体
Androgen, oestrogen and progesterone receptors
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
相关类别
细胞系来源
Mouse BALB/c testis sertoli cell
细胞系描述
From primary culture of sertoli cell enriched preparations from normal testes of a 11-13 day old BALB/c nu/+ mice. Cells respond to FSH but not LH; Plasminogen activator secretion is stimulated by FSH and also retinoic acid. Note: only distributed frozen.
应用
Hormone studies
培养基
Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:20 to 1:200 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cells resuscitated from frozen stock should be split 1:3 at the first passage and thereafter as above.
其他说明
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法规信息
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