生物来源
human bone marrow
生长模式
Suspension
核型
2n = 46
形态学
Lymphoblast
产品
Immunoglobulin G (IgG)
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
相关类别
细胞系来源
Human B lymphocyte
细胞系描述
Derived from bone marrow removed from a female patient with multiple myeloma. The cells synthesise IgG at a rate of 2.4ug/1,000,000 cells/day and have receptors for insulin, calcitonin and human growth hormone. They also exhibit surface markers and receptor sites characteristic of B lymphocytes.
应用
Biosynthetic and surface marker studies
培养基
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Maintain cultures between 2-4x100,000 cells/ml; 5% CO2; 37°C. Maximum cell density 1,000,000 cells/ml.
其他说明
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