CCRF-CEM

A T lymphoblastoid line obtained from the peripheral blood of a 4 year old Caucasian female with acute lymphoblastoid leukaemia. These may be grown to a high density in a spinner type suspension culture. Cells are reported to be free of virus particles as determined by electron microscopy.

Human Caucasian acute lymphoblastic leukaemia

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,12
D16S539: 10,13
D5S818: 12,13
D7S820: 9,13
THO1: 6,7
TPOX: 8
vWA: 17,19

Maintain cultures between 2-3x100,000 cells/ml; 5% CO₂; 37°C. Maximum cell density 1-2x1,000,000 cells/ml.

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.