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生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
ascites fluid
抗体产品类型
primary antibodies
克隆
NCAM-0B11, monoclonal
分子量
antigen 140-180 kDa
包含
15 mM sodium azide
种属反应性
rat, human
技术
immunocytochemistry: suitable using cells and tissues
western blot: 1:100 using a freshly prepared extract from newborn and adult rat brain, or rat cerebral cortex extract
同位素/亚型
IgG1
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... NCAM1(4684) , NCAM2(4685)
rat ... Ncam1(24586) , Ncam2(288280)
一般描述
Monoclonal Anti-N-CAM (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Neural cell adhesion molecule (N-CAM), the best characterized CAM, exists in adult brain. It belongs to the family of sialoglycoproteins, which arise from alternative splicing of mRNA transcribed from a single gene. Immunocytochemical data indicate that in the adult, N-CAM is expressed mainly in cells of the nervous system. There are indications that N-CAM may also be expressed by non-neural cells in the adult, as it has been found in studies of various embryonic developmental stages.
Mouse monoclonal clone NCAM-0B11 anti-Neural Cell Adhesion Molecule antibody localizes the high molecular weight isoform of N-CAM (neural cell adhesion molecule) in human and several other mammalian species. The antibody shows a strong reaction to N-CAM A/N-CAM 180, however it also recognizes N-CAM B/N-CAM 140. In an immunoblot, the product reacts with polysialated N-CAM in embryonic material. Breakdown products of N-CAM may be stained; they appear as bands lower than 100 kD on the immunoblot.
免疫原
growth cone enriched plasma membrane fraction from E17 rat forebrain.
应用
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (3 papers)
Immunofluorescence (1 paper)
Immunohistochemistry (3 papers)
Monoclonal Anti-Neural Cell Adhesion Molecule antibody produced in mouse has been used in:
- Immunofluorescence
- Immunohistochemistry
- Immunoblotting
生化/生理作用
Neural cell adhesion molecule (N-CAM) are believed to be involved in cell-cell interactions and probably play an important role in embryogenesis and development.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Neural cell adhesion molecule (NCAM) isoform expression is associated with neuroblastoma differentiation status
Winter C, et al.
Pediatric Blood & Cancer, 51(1), 10-16 (2008)
Expression and localization of neural cell adhesion molecule and polysialic acid during chick corneal development
Mao X, et al.
Investigative Ophthalmology & Visual Science, 53(3), 1234-1243 (2012)
Kif3a controls murine nephron number via GLI3 repressor, cell survival, and gene expression in a lineage-specific manner
Chi L et al.
Testing, 8(6), e65448-e65448 (2013)
The ubiquitous neural cell adhesion molecule (N-CAM)
Weledji EP and Assob JC
Annals of medicine and surgery, 3(3), 77-81 (2014)
Nephron Progenitor But Not Stromal Progenitor Cells Give Rise to Wilms Tumors in Mouse Models with
Huang L, et al.
Neoplasia, 18(2), 71-81 (2016)
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