C9281
胆固醇酯酶 来源于荧光假单胞菌
lyophilized powder, ≥10,000 units/g protein
别名:
CE, 胆固醇酯水解酶, 胆盐刺激性脂, 胰胆固醇酯酶, 甾醇酯酰水解酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-808-6
MDL number:
Specific activity:
≥10,000 units/g protein
Assay:
10-30% (TCA-Biuret)
Biological source:
Pseudomonas fluorescens
biological source
Pseudomonas fluorescens
assay
10-30% (TCA-Biuret)
form
lyophilized powder
specific activity
≥10,000 units/g protein
mol wt
~129 kDa
composition
Protein, ~20%
storage condition
under inert gas (argon)
technique(s)
cell based assay: suitable
color
tan to brown
pH
7-9
solubility
0.4 M potassium phosphate, pH 7.0: soluble 1.0 mg/mL
storage temp.
−20°C
Quality Level
General description
研究领域：细胞信号转导
胆固醇酯酶(CE)是一种糖蛋白，可从柱状假丝酵母和荧光假单胞菌等真菌中提取。属于脂肪酶/酯酶家族成员，以同型二聚体形式发挥作用。胆固醇酯酶在胰腺中生成，经胆囊收缩素(CCK)刺激后以活性形式释放。
胆固醇酯酶(CE)是一种糖蛋白，可从柱状假丝酵母和荧光假单胞菌等真菌中提取。属于脂肪酶/酯酶家族成员，以同型二聚体形式发挥作用。胆固醇酯酶在胰腺中生成，经胆囊收缩素(CCK)刺激后以活性形式释放。
Application
胆固醇酯酶（来自荧光假单胞菌）用于：
- 在胆固醇酯酶检测中用于定量分析人血清样本中的总胆固醇
- 脂解制备物酯酶活性的非变性蛋白电转移研究
- 酶法胆固醇测定试剂（含胆固醇氧化酶）的组分优化研究
- 修饰人血浆低密度脂蛋白(LDL)，用于在分支组织工程血管(TEBV)中诱导内皮细胞(EC)障碍和单核细胞黏附(MC)
- 在菲律宾菌素染色法检测视网膜冷冻切片CE中，水解天然胆固醇酯(CE)
该酶广泛应用于诊断实验室血清胆固醇的测定。
Biochem/physiol Actions
胆固醇酯酶(CE)是一种可水解或合成胆固醇等固醇物质脂肪酸酯部分的可逆性酶。胆固醇酯酶活性在类固醇生物合成或类固醇从头合成(de novo steroidogenesis)中起重要作用。对水不溶性长链脂肪酸酯的水解需要胆汁盐激活。对水溶性短链脂肪酸酯和溶血磷脂的水解则不需要胆汁盐激活。还可水解甘油三酯、甘油二酯和甘油一酯、磷脂、溶血磷脂和神经酰胺。该酶在脂质和脂蛋白代谢以及动脉粥样硬化中起多种作用。
Analysis Note
双缩脲法测定蛋白质。
Other Notes
含有磷酸钾和 TRITON®X-100。
在牛磺胆酸盐存在下，一个单位在 pH7.0，37°C 下每分钟将 1.0μ 摩尔胆固醇油酸酯水解成胆固醇和油酸。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
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此项目有
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商品
Cholesterol esterification enhances transport efficiency in lipoproteins for increased blood stream transport.
胆固醇酯化可改善运输。胆固醇酯可更轻松地封装在脂蛋白内部-增加了在血液中可轻松运输的胆固醇数量。
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Instructions
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