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C9210

Sigma-Aldrich

溴化氰活化的琼脂糖

lyophilized powder

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别名:
Agarose, cyclic carbonimidate, CNBr-Activated Agarose
CAS号:
68987-32-6
MDL编号:
MFCD00163385
UNSPSC代码:
41106500
NACRES:
NA.56

形式

lyophilized powder

质量水平

200

技术

affinity chromatography: suitable

基质

cross-linked 4% beaded agarose

基质活性基团

cyanate or related structures

基质隔离区

1 atom (when ligands are coupled via isourea derivative or related linkage)

溶胀

1 g swells to 2.5-4.5 mL

容量

≥10 mg/mL binding capacity (BSA)(via amino groups)

适用性

suitable for chromatography

储存温度

−20°C

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应用

氰化溴化物活化的琼脂糖是一种用乳糖稳定的冻干粉，可用于亲和层析、蛋白层析、蛋白相互作用、抗体标记、抗体修饰以及将抗体附着于琼脂糖珠上。

外形

用乳糖稳定化的冻干粉

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What do you recommend to use as a negative control for Product C9210, Cyanogen bromide?

    The control for this product is Product No. CL4B200 (Sepharose CL-4B).

  4. What is the difference between Product C9210, Cyanogen bromide and Product C9142, Cyanogen bromide-activated-Sepharose® 4B?

    Sigma-Aldrich produces product C9210, while C9142 is produced by GE Healthcare.  In addition, C9210 is a cross-linked resin and C9142 is not.

  5. When using Product C9210, Cyanogen bromide, why must amine containing reagents be avoided during coupling?

    The ligand is attached to the resin via an amine group. If an amine-containing buffer is used during coupling, you will greatly reduce the amount of ligand that can attach.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

R A Gilissen et al.
Biochemical pharmacology, 43(12), 2661-2663 (1992-06-23)
A method for the covalent binding of rat liver UDP-glucuronosyltransferase to a cyanogen bromide-activated agarose matrix is described. The rat liver microsomal fraction was solubilized with 8 mM 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS); 90% of the microsomal protein was solubilized. Some 50-60% of
Mi Young Cha et al.
Bioconjugate chemistry, 22(1), 88-94 (2010-12-15)
We developed fluorescent biosensor systems that are either general or selective to fluoroquinolone antibiotics by using a single-chain variable-fragment (scFv) as a recognition element. The selectivity of these biosensors to fluoroquinolone antibiotics was rationally tuned through the structural modification on
N A Morjana et al.
Protein expression and purification, 5(2), 144-148 (1994-04-01)
Protein disulfide isomerase (PDI) catalyzes the formation and rearrangement of disulfide bonds during protein folding. PDI coupled to cyanogen bromide-activated agarose retains its catalytic activity, and a column of this material increases both the rate and the yield for folding
Rodney D Franklin et al.
Obstetrics and gynecology, 101(3), 455-462 (2003-03-15)
To compare the efficacy of unfractionated heparin and low molecular weight heparin in the in vitro binding of antiphospholipid antibodies obtained from the sera of patients with recurrent pregnancy loss. Women with immunoglobulin (Ig) G antibodies to the phospholipids cardiolipin
DamIP: Using Mutant DNA Adenine Methyltransferase to Study DNA-Protein Interactions <I>In Vivo</I>.
Xiao, R., and Moore, D.D.
Current Protocols in Molecular Biology, 94, 21-21 (2011)
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