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重组
expressed in Streptomyces lividans
质量水平
表单
lyophilized solid
比活
≥18 unit/mg solid
分子量
~37 kDa by SDS-PAGE
储存温度
−20°C
应用
此胆固醇脱氢酶的序列来自诺卡氏菌(Nocardia sp.),通过变铅青链霉菌(Streptomyces lividans)表达。胆固醇脱氢酶（诺卡氏菌）已用于制备安培型胆固醇生物传感器，检测生物样品中的胆固醇，并用于研究常染色体隐性遗传性高胆固醇血症磷酸酪氨酸结合域与低密度脂蛋白(LDL)受体尾部复合体的原子结构。胆固醇脱氢酶（诺卡氏菌）还用于研究Ras纳米簇在膜结构域中的组织、动态和解离。
用于测定血清胆固醇的酶
生化/生理作用
胆固醇脱氢酶(CDH)是NAD(P)依赖性微生物脱氢酶，可氧化胆固醇的3-β-羟基生成胆甾烯-4-烯-3-酮。SDS-PAGE法测定的蛋白分子量为37kDa。pH是4.5。
胆固醇脱氢酶(CDH)是依赖烟酰胺腺嘌呤二核苷酸磷酸(NAD(P))的微生物脱氢酶，可氧化胆固醇的3-β-羟基生成胆甾烯-4-烯-3-酮。相比基于胆固醇氧化酶的分析方法，CDH可更加轻松地直接定量测定胆固醇。
单位定义
一个单位将在pH 8.5和25℃下，每分钟产生1.0 μmole胆甾烯-4-烯-3-酮。
外形
以冻干粉形式提供
警示用语：
Danger
危险声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Cloning, nucleotide sequence, and transcriptional analysis of the NAD (P)-dependent cholesterol dehydrogenase gene from a Nocardia sp. and its hyperexpression in Streptomyces spp.
Horinouchi S, et al.
Applied and Environmental Microbiology, 57(5) (1991)
A disposable amperometric biosensor for determining total cholesterol in whole blood
Fang C, et al.
Sensors and Actuators B, Chemical, 155(2) (2011)
Takeshi Nomura et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(22), 8770-8775 (2012-05-16)
Mechanosensitive (MS) channels of small (MscS) and large (MscL) conductance are the major players in the protection of bacterial cells against hypoosmotic shock. Although a great deal is known about structure and function of these channels, much less is known
Lorant Janosi et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(21), 8097-8102 (2012-05-09)
Recent experiments have shown that membrane-bound Ras proteins form transient, nanoscale signaling platforms that play a crucial role in high-fidelity signal transmission. However, a detailed characterization of these dynamic proteolipid substructures by high-resolution experimental techniques remains elusive. Here we use
The characteristics and applications of recombinant cholesterol dehydrogenase
Kishi K, et al.
Bioscience, Biotechnology, and Biochemistry, 64(7) (2000)
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