一般描述
这种人重组蛋白是从感染含有人细胞色素 P450 还原酶 cDNA 的杆状病毒的昆虫细胞中分离得到的。通过亲和层析纯化。
应用
sigma 的酶已用于抗癌前体药物 1,2-双（甲磺酰基）-1-（2-氯乙基）-2-[[1-（4-硝基苯基）乙氧基] 羰基] 肼 (KS119) 的低氧和有氧还原。
在一项研究中使用人细胞色素 P450 还原酶评估去乙酰化酶抑制剂 panobinostat (LBH589) 环化代谢物的生物催化合成和结构解析。人细胞色素 P450 还原酶也被用于研究耦合运动对沿人微粒体 P450 链的电子的影响。
NADPH-P450 还原酶是一种膜结合黄素蛋白，可将电子从 NADPH 转移到细胞色素 P450 的各种亚型。通常使用的还原酶：P450 的比例为 0.5-5:1。这种酶每摩尔蛋白质中含有一摩尔 FAD 和 FMN。
生化/生理作用
细胞色素 P450 还原酶是微粒体中电子从 NADP 转移到细胞色素 P450 所需的膜结合酶。也能提供电子转移给血红素加氧酶和细胞色素 B5。细胞色素 P450 酶系统主要参与肝脏对外源物质的解毒。也参与前致癌物的激活和内源性底物如类固醇的代谢。
单位定义
一个单位将导致在 pH7.4、37 ℃下每分钟用 NADPH 还原 1.0 μmol 细胞色素c。
外形
在含有 10 mM 磷酸钾、pH 7.4、0.1 mM EDTA、0.5 mM DTT、20% (v/v) 甘油的溶液中提供
WGK
WGK 2
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
Bas van de Waterbeemd et al.
PloS one, 8(5), e65157-e65157 (2013-06-07)
An improved detergent-free process has been developed to produce vaccine based on native outer membrane vesicles (NOMV) against Neisseria meningitidis serogroup B. Performance was evaluated with the NonaMen vaccine concept, which provides broad coverage based on nine distinct PorA antigens.
Y Yasukochi et al.
The Journal of biological chemistry, 251(17), 5337-5344 (1976-09-10)
NADPH-cytochrome c (cytochrome P-450) reductase (EC 1.6.2.4) has been purified to homogeneity, as judged by sodium dodecyl sulfate disc gel electrophoresis, from detergent-solubilized rat and pig liver microsomes using an affinity chromatography procedure. Treatment of microsomes with a polyethoxynonylphenyl ether
Jiechuang Su et al.
The Biochemical journal, 452(1), 79-86 (2013-03-14)
One-electron reductases that reduce nitro compounds in hypoxic human tumour cells are poorly characterized, but are important for targeting hypoxia with nitroaromatic prodrugs. Fluorogenic probes with defined reductase profiles are needed to interrogate the activity of these enzymes in intact
J H Convery et al.
Physical review. E, Statistical, nonlinear, and soft matter physics, 86(1 Pt 1), 011903-011903 (2012-09-26)
The conditions necessary for the formation of a monolayer and a bilayer of a mutated form (P499C) of human cytochrome P450 reductase on a Au(110)/electrolyte interface have been determined using a quartz crystal microbalance with dissipation, atomic force microscopy, and
Chunja Lee et al.
Drug metabolism and disposition: the biological fate of chemicals, 41(10), 1782-1786 (2013-07-13)
The aryl hydrocarbon receptor (AHR)-dependent induction of cytochromes P450 (P450) such as CYP1A1 by 3-methylcholanthrene (MC) and related polycyclic aromatic hydrocarbons is well characterized. We reported previously that MC treatment triggers a pronounced downregulation, particularly at the protein level, of
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