应用
来自Sigma的酶已用于分析硫酸皮肤素十二糖中艾杜糖醛酸的频率。它也被用于消化黑素瘤细胞中的硫酸皮肤素（DS）。这种消化导致癌细胞的增殖和侵袭性降低，从而显示了DS在转移中的作用。
来自肝素黄杆菌的软骨素酶B已用于评估硫酸皮肤素的结构表征和抗凝血酶活性的研究中。肝素黄杆菌的软骨素酶B也已用于通过液相色谱-质谱法研究软骨素裂解酶的作用方式的研究中。
生化/生理作用
软骨素酶B仅降解硫酸软骨素B，产生寡糖和四糖，以及不饱和的4-硫酸二糖。它的最佳温度为20℃，最佳pH值为8.0。使用0.1 M NaCl可将酶活性抑制50%。Co2+、Fe3+和Ba2+也抑制其活性。
单位定义
使用硫酸软骨素B作为底物，在25℃和pH 7.5下，一个单位每小时将形成0.1 μmole不饱和糖醛酸。
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
含少量动物源组分生物产品
常规特殊物品
Zhenqing Zhang et al.
Analytical biochemistry, 385(1), 57-64 (2008-11-11)
Liquid chromatography-mass spectrometry was applied to determine the action pattern of different chondroitin lyases. Two commercial enzymes, chondroitinase ABC (Proteus vulgaris) and chondroitinase ACII (Arthrobacter aurescens), having action patterns previously determined by viscosimetry and gel electrophoresis were first examined. Next
Yvette M Coulson-Thomas et al.
Journal of neuroscience methods, 171(1), 19-29 (2008-04-18)
Injury to the CNS of vertebrates leads to the formation of a glial scar and production of inhibitory molecules, including chondroitin sulphate proteoglycans. Various studies suggest that the sugar component of the proteoglycan is responsible for the inhibitory role of
Y M Michelacci et al.
The Biochemical journal, 151(1), 121-129 (1975-10-01)
A chondroitinase that degrades only chondroitin sulphate B was isolated from Flavobacterium heparinum, and separated from a constitutive chondroitinase AC also present in extracts of F. heparinum. The enzyme acts only on chondroitin sulphate B, producing oligo- and tetra-saccharides, plus
Nicola Volpi et al.
Glycobiology, 19(4), 356-367 (2008-12-06)
Glycosaminoglycans from the body of marine clam Scapharca inaequivalvis were extracted at about 0.15- 0.18 mg/g of dry tissue, composed of dermatan sulfate (DS) (approx. 74%) and heparan sulfate (26%). After treatment with nitrous acid, DS was isolated for further
Valentina Gargiulo et al.
Glycobiology, 19(12), 1485-1491 (2009-08-22)
Chondroitin sulfate (CS), a constituent of proteoglycans, is a key component of the connective tissues and it is widely used as a precautionary drug for joint diseases; for this reason, the increased demand of this polysaccharide has posed the problem
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Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
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